ASAH1 Antibody (1A7) - Azide and BSA Free

Western Blot: ASAH1 Antibody (1A7) [H00000427-M02] - Analysis of ASAH1 expression in transfected 293T cell line by ASAH1 monoclonal antibody (M02), clone 1A7.Lane 1: ASAH1 transfected lysate(45 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: ASAH1 Antibody (1A7) [H00000427-M02] - Analysis of monoclonal antibody to ASAH1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.

ELISA: ASAH1 Antibody (1A7) [H00000427-M02] - Detection limit for recombinant GST tagged ASAH1 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ASAH1 Antibody (1A7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ASAH1 (NP_808592, 25 a.a. ~ 124 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPMLKVIVNSLKNMINTFVPSGKVMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTD
• Specificity: ASAH1 - N-acylsphingosine amidohydrolase (acid ceramidase) 1 (1A7)
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ASAH1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been used for IHC-P.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ASAH1 Antibody (1A7) - Azide and BSA Free

• ACDase
• ACEC 3.5.1.23
• Acid CDase
• acid ceramidase
• Acylsphingosine deacylase
• ASAH
• FLJ21558
• FLJ22079
• N-acylsphingosine amidohydrolase (acid ceramidase) 1
• N-acylsphingosine amidohydrolase
• PHP
• PHP32N-acylsphingosine amidohydrolase (acid ceramidase)
• Putative 32 kDa heart protein

Background

This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASAH1
• Entrez:
  • 427(Human)
• Uniprot:
  • NP_808592(Human)