ASF1a Antibody (2A5F8) - BSA Free

Western Blot: ASF1a Antibody (2A5F8) [NBP2-61683] - Analysis using ASF1A mAb against human ASF1A (AA: 1-204) recombinant protein. (Expected MW is 48.9 kDa)

Flow Cytometry: ASF1a Antibody (2A5F8) [NBP2-61683] - Analysis of Hela cells using ASF1A mouse mAb (green) and negative control (red).

Western Blot: ASF1a Antibody (2A5F8) [NBP2-61683] - Analysis using ASF1A mAb against HEK293 (1) and ASF1A (AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: ASF1a Antibody (2A5F8) [NBP2-61683] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: 2A5F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

ASF1a Antibody (2A5F8) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human ASF1a (AA: 1-204) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ASF1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Western Blot 1:100-1:2000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for ASF1a Antibody (2A5F8) - BSA Free

• ASF1 silencing function 1 homolog A (S. cerevisiae)
• CCG1-interacting factor A
• CIACGI-98
• DKFZP547E2110
• hAsf1
• hAsf1a
• hCIA
• histone chaperone ASF1A
• HSPC146
• silencing function 1A
• silencing function protein 1 homolog A

Background

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASF1A