ATP Assay Kit (Colorimetric/Fluorometric)

Product Discontinued

ATP Assay Kit (Colorimetric/Fluorometric) Summary

• Description: Kit is designed to be a robust method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified.
  
  Detection method: Fluorescence plate reader (Ex/Em 535/587). Absorbance at 570 nmApplications: The assay can detect as low as 50 picomol (1 UM) of ATP in various samples.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab83355)
• Kit Type: Assay Kit (Colorimetric/Fluorometric)

Applications/Dilutions

Publications

Read Publication using NBP2-54855.

Packaging, Storage & Formulations

• Storage: Store at -20C.

Background

ATP is the primary energy currency of living systems. Virtually all energy requiring processes utilize the chemical energy stored in the phosphate bond of ATP. ATP is formed exclusively in the mitochondria and a variety of genetic diseases can affect ATP formation. There are a number of commercially available ATP assays which detect femtomoles or less of ATP by measuring luminescence (Novus Kit 254-200, for example) but these kits require specialized luminescence instrumentation and utilize luciferase which can be difficult to maintain in active form. Novus' ATP Colorimetric and Fluorometric Assay kit is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (WLmax = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for ATP Kit (NBP2-54855)(1)

Publication using NBP2-54855

• Foresti R, Rossi S, Pinelli S et al. In-vivo vascular application via ultra-fast bioprinting for future 5D personalised nanomedicine Sci Rep 2020-02-21 [PMID: 32081937]