Recombinant Human Aurora B His Protein

SDS-Page: Recombinant Human Aurora B His Protein [NBP1-30242] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

SDS-Page: Recombinant Human Aurora B His Protein [NBP1-30242] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human Aurora B His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1- 344 of Human Aurora B
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAQKENSYPW PYGRQTAPSG LSTLPQRVLR KEPVTPSALV LMSRSNVQPT AAPGQKVMEN SSGTPDILTR HFTIDDFEIG RPLGKGKFGN VYLAREKKSH FIVALKVLFK SQIEKEGVEH QLRREIEIQA HLHHPNILRL YNYFYDRRRI YLILEYAPRG ELYKELQKSC TFDEQRTATI MEELADALMY CHGKKVIHRD IKPENLLLGL KGELKIADFG WSVHAPSLRR KTMCGTLDYL PPEMIEGRMH NEKVDLWCIG VLCYELLVGN PPFESASHNE TYRRIVKVDL KFPASVPMGA QDLISKLLRH NPSERLPLAQ VSAHPWVRAN SRRVLPPSAL QSVA

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: AURKB
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 41.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 0.5 MM DTT, 20% glycerol, 0.1 mM EDTA, 0.1 mM EGTA, 0.1 M NaCl, 0.1 mM PMSF
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Aurora B His Protein

• Aik2
• AIK2EC 2.7.11.1
• AIM1
• AIM-1
• AIM1Aik2
• AIM-1STK-1
• ARK2
• ARK2ARK-2
• AurB
• AURKB
• aurkb-sv2
• Aurora- and IPL1-like midbody-associated protein 1
• Aurora B
• aurora kinase BSerine/threonine-protein kinase aurora-B
• aurora kinase B-Sv1
• aurora kinase B-Sv2
• Aurora/IPL1-related kinase 2
• aurora-1
• aurora-B
• Aurora-related kinase 2
• EC 2.7.11
• IPL1
• serine/threonine kinase 12
• serine/threonine-protein kinase 12
• STK12
• STK12aurkb-sv1
• STK5

Background

Aurora Kinase B is a member of a family of mitotic serine/threonine kinases. Aurora Kinase B associates with chromosomes during prophase prior to relocalizing to the spindle at anaphase. Aurora Kinase B regulates chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. Expression of Aurora Kinase B during the G2/M phase transition is tightly coordinated with histone H3 phosphorylation, while overexpression in many kinds of cancers. Recombinant human Aurora Kinase B, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: AURKB