B-Myb Antibody

Western Blot: B-Myb Antibody [NB100-53779] - Analysis of rat stomach lysate

• Reactivity: Rt, Hu, Mu, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

B-Myb Antibody Summary

• Immunogen: Peptide with sequence C-QEKARQLLGRLKPSH corresponding to C-Terminus according to NP_002457.1.
• Predicted Species: Human (100%), Mouse (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MYBL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 100 kDa band observed in rat stomach lysates (calculated MW of 78.8 kDa band according to human NP_002457.1).
• Control:

  B-Myb Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for B-Myb Antibody

• B-MYB
• BMYBMGC15600
• Myb-like protein 2
• myb-related protein B
• v-myb avian myeloblastosis viral oncogene homolog-like 2
• v-myb myeloblastosis viral oncogene homolog (avian)-like 2

Background

The protein encoded by this gene, a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. The encoded protein is phosphorylated by cyclin A/cyclin-dependent kinase 2 during the S-phase of the cell cycle and possesses both activator and repressor activities. It has been shown to activate the cell division cycle 2, cyclin D1, and insulin-like growth factor-binding protein 5 genes. Transcript variants may exist for this gene, but their full-length natures have not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B-Myb Antibody (NB100-53779)(1)

Publication using NB100-53779

• Shepard JL, Amatruda JF, Stern HM et al. A zebrafish bmyb mutation causes genome instability and increased cancer susceptibility. Proc Natl Acad Sci U S A 2005-09-13 [PMID: 16150706]

Bioinformatics

• Gene Symbol: MYBL2
• Entrez:
  • 4605(Human)
  • 17865(Mouse)
  • 296344(Rat)
• Uniprot:
  • NP_002457.1(Human)