B7-H7/HHLA2 Antibody (907812) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human B7-H7/HHLA2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841) followed by anti-Mouse IgG APC-conjugated secondary antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

B7-H7/HHLA2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

In a functional flow cytometry test, biotinylated recombinant human TMIGD2/CD28H (10 ng/mL, Catalog # 8316-TR) binds to HEK293 human embryonic kidney cell line transfected with human B7-H7/HHLA2 (black dotted line). Binding is completely blocked (orange histogram) by 2.5 μg/mL of Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841). Mouse IgG1 Isotype Control (Catalog # MAB002) at 2.5 μg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050).

In a Surface Plasmon Resonance test, Recombinant Human TMIGD2/CD28H Fc Chimera (Catalog # 8316-TR), coated at 10 ng/mL binds to Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7, 5 μg/mL) (blue line). Binding is completely blocked (red) by 50 μg/mL of Mouse Anti-Human B7-H7/HHLA2 Monoclonal Antibody (Catalog # MAB80841). Mouse IgG1 isotype (Catalog # MAB002) and irrelevant mouse monoclonal antibody were used as controls and did not affect TMIGD2/CD28H binding to B7-H7/HHLA2 (not shown).

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready, ICC/IF
• Clone: 907812
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

B7-H7/HHLA2 Antibody (907812) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived human B7-H7/HHLA2
  Met1-Asn344
  Accession # Q9UM44
• Specificity: Detects human B7-H7/HHLA2 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HHLA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction 100-300 ng/mL
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for B7-H7/HHLA2 Antibody (907812) [Unconjugated]

• B7H7
• B7-H7
• HERV-H LTR-associating 2
• HERV-H LTR-associating protein 2
• HHLA2
• Human endogenous retrovirus-H long terminal repeat-associating protein 2

Background

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5).
B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4⁺ and CD8⁺ T cells and their production of IFN-gamma , TNF-alpha , IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).

1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
3. Mager, D.L. et al. (1999) Genomics 59:255.
4. Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
5. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for B7-H7/HHLA2 Antibody (MAB80841)(1)

Publication using MAB80841

• Xiaoxuan Zhuang, Eric O. Long CD28 Homolog Is a Strong Activator of Natural Killer Cells for Lysis of B7H7+ Tumor Cells Cancer Immunology Research 2019-06-01 [PMID: 31018957]

Bioinformatics

• Gene Symbol: HHLA2
• Uniprot:
  • Q9UM44()