BACE-1 Antibody (137612)

Western blot shows lysates of human brain (Alzheimer's disease hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse BACE-1 Ectodomain Monoclonal Antibody (Catalog # MAB931) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for BACE-1 at approximately 60 and 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of human brain (Alzheimer's disease hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse BACE-1 Ectodomain Monoclonal Antibody (Catalog # MAB931) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for BACE-1 at approximately 60 and 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB, IHC, CyTOF-ready, Flow
• Clone: 137612
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH
• Suitable Sample Type: 23038

BACE-1 Antibody (137612) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BACE-1
  Thr22-Tyr460
  Accession # P56817
• Specificity: Detects human and mouse BACE-1 in direct ELISAs and human BACE-1 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) BACE-2, recombinant mouse (rm) BACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BACE1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunohistochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BACE-1 Antibody (137612)

• APP beta-secretase
• ASP2
• Aspartyl protease 2
• BACE1
• BACE-1
• BACEAsp 2
• beta-secretase 1 precursor variant 1
• beta-secretase 1
• beta-site amyloid beta A4 precursor protein-cleaving enzyme
• Beta-site amyloid precursor protein cleaving enzyme 1
• Beta-site APP cleaving enzyme 1
• beta-site APP-cleaving enzyme 1
• beta-site APP-cleaving enzyme
• EC 3.4.23
• EC 3.4.23.46
• FLJ90568
• HSPC104
• KIAA1149
• memapsin-2
• Membrane-associated aspartic protease 2
• transmembrane aspartic proteinase Asp2

Background

BACE-1 (beta‑site APP cleaving enzyme-1) is an aspartic protease and an integral membrane protein (1, 2). It is the major beta secretase, and together with the gamma  secretase, is responsible for generating the amyloid beta peptide (A beta ) from the amyloid precursor protein (APP) (3, 4). Because A beta is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. High levels of BACE-1 activity are sufficient to elicit neurodegeneration and neurological decline in vivo, indicating that inhibiting BACE-1 may block not only A beta -dependent but also A beta -independent pathogenic mechanisms (5). In addition to APP, BACE-1 also cleaves APP-like proteins 1 and 2, the cell adhesion protein P-selectin glycoprotein ligand-1 and beta -galactoside alpha 2,6-sialyltransferase, implying that BACE-1 may have additional functions involving the ectodomain shedding of membrane proteins (6‑8).

1. Vassar, R. et al. (1999) Science 286:735.
2. Yan, R. et al. (1999) Nature 402:533.
3. Cai, H. et al. (2001) Nature Neurosci. 4:233.
4. Roberds, S.L. et al. (2001) Human Mol. Genet. 97:1317.
5. Rockenstein, E. et al. (2005) J. Biol. Chem. 280:32957.
6. Li, Q and T.C. Sudhof (2004) J. Biol. Chem. 279:10542.
7. Lichtenthaler, S.F. et al. (2003) J. Biol. Chem. 278:48713.
8. Kitazynem, S. et al. (2005) J. Biol. Chem. 280:8589.

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Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BACE-1 Antibody (MAB931)(15)

Publications using MAB931

• Mariko Sawa, Cassia Overk, Ann Becker, Dominique Derse, Ricardo Albay, Kim Weldy et al. Impact of increased APP gene dose in Down syndrome and the Dp16 mouse model Alzheimer's & Dementia 2022-06-01 [PMID: 34757693]
• MI Khan, JH Shin, MY Kim, TS Shin, JD Kim Green Tea Seed Isolated Theasaponin E1 Ameliorates AD Promoting Neurotoxic Pathogenesis by Attenuating Abeta Peptide Levels in SweAPP N2a Cells Molecules, 2020-05-16;25(10):. 2020-05-16 [PMID: 32429462] (Western Blot, Mouse)
• J Wang, A Wuethrich, AA Sina, RE Lane, LL Lin, Y Wang, J Cebon, A Behren, M Trau Tracking extracellular vesicle phenotypic changes enables treatment monitoring in melanoma Sci Adv, 2020-02-26;6(9):eaax3223. 2020-02-26 [PMID: 32133394] (Western Blot, Human)
• A Mendsaikha, I Tooyama, JP Bellier, GE Serrano, LI Sue, LF Lue, TG Beach, DG Walker Characterization of lysosomal proteins Progranulin and Prosaposin and their interactions in Alzheimer's disease and aged brains: increased levels correlate with neuropathology Acta Neuropathol Commun, 2019-12-21;7(1):215. 2019-12-21 [PMID: 31864418] (IHC-Fr, Western Blot, Human)
• Yong Shen, Haibo Wang, Qiying Sun, Hailan Yao, Andrew P. Keegan, Mike Mullan et al. Increased Plasma Beta-Secretase 1 May Predict Conversion to Alzheimer’s Disease Dementia in Individuals With Mild Cognitive Impairment Biological Psychiatry 2018-03-01 [PMID: 28359566]
• Schnoder L, Hao W, Qin Y, Liu S, Tomic I, Liu X, Fassbender K, Liu Y Deficiency of Neuronal p38alpha MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1. J Biol Chem, 2015-12-09;291(5):2067-79. 2015-12-09 [PMID: 26663083] (IHC, Western Blot, Human)
• Wang Y, Thinakaran G, Kar S Overexpression of the IGF-II/M6P receptor in mouse fibroblast cell lines differentially alters expression profiles of genes involved in Alzheimer's disease-related pathology. PLoS ONE, 2014-05-20;9(5):e98057. 2014-05-20 [PMID: 24846272] (Western Blot, Mouse)
• Cheng X, He P, Lee T, Yao H, Li R, Shen Y High activities of BACE1 in brains with mild cognitive impairment. Am J Pathol, 2014-01-01;184(1):141-7. 2014-01-01 [PMID: 24332014] (ELISA Development, Western Blot, Human)
• Ping He, Pengtao Li, Qian Hua, Yuan Liu, Matthias Staufenbiel, Rena Li et al. Chronic Administration of Anti-Stroke Herbal Medicine TongLuoJiuNao Reduces Amyloidogenic Processing of Amyloid Precursor Protein in a Mouse Model of Alzheimer’s Disease PLoS ONE 2013-03-05 [PMID: 23472157]
• Ping He, Xin Cheng, Matthias Staufenbiel, Rena Li, Yong Shen Long-Term Treatment of Thalidomide Ameliorates Amyloid-Like Pathology through Inhibition of beta -Secretase in a Mouse Model of Alzheimer’s Disease PLoS ONE 2013-02-06 [PMID: 23405115]
• Amanda Gonzales, Boris Decourt, Aaron Walker, Rachel Condjella, Hikmet Nural, Marwan N. Sabbagh Development of a specific ELISA to measure BACE1 levels in human tissues Journal of Neuroscience Methods 2011-10-30 [PMID: 21889954]
• Yan-Jiang Wang, Xin Wang, Jian-Jun Lu, Qiao-Xin Li, Chang-Yue Gao, Xiao-Hong Liu et al. p75NTR regulates Abeta deposition by increasing Abeta production but inhibiting Abeta aggregation with its extracellular domain The Journal of Neuroscience 2011-02-09 [PMID: 21307265]
• M. Paul Murphy, Jacqueline Morales, Tina L. Beckett, Giuseppe Astarita, Daniele Piomelli, Adam Weidner et al. Changes in Cognition and Amyloid-beta Processing with Long Term Cholesterol Reduction using Atorvastatin in Aged Dogs Journal of Alzheimer's Disease 2010-09-28 [PMID: 20847439]
• Viorela Pop, Elizabeth Head, Nicole C. Berchtold, Charles G. Glabe, Christa M. Studzinski, Adam M. Weidner et al. A beta aggregation profiles and shifts in APP processing favor amyloidogenesis in canines Neurobiology of Aging 2012-01-01 [PMID: 20434811]
• Ahmed RR, Holler CJ, Webb RL BACE1 and BACE2 enzymatic activities in Alzheimer's disease. J. Neurochem., 2009-12-04;112(4):1045-53. 2009-12-04 [PMID: 19968762] (ELISA Development, Immunoprecipitation, Western Blot, Human, Rat)

Bioinformatics

• Gene Symbol: BACE1
• Uniprot:
  • P56817()