Bassoon Antibody

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Bassoon Antibody Summary

• Immunogen: A synthetic peptide from internal region of human Bassoon conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BSN
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for Bassoon Antibody

• bassoon (presynaptic cytomatrix protein)

Background

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bassoon Antibody (NBP1-46351)(1)

Publication using NBP1-46351

• Shelkar GP, Liu J, Dravid SM Astrocytic NMDA receptors in the basolateral amygdala contribute to facilitation of fear extinction The international journal of neuropsychopharmacology 2021-08-07 [PMID: 34363482] (IF/IHC, Mouse)

FAQs for Bassoon Antibody (NBP1-46351). (Showing 1 - 2 of 2 FAQ).

1. I am looking for a good antibody to detect Bassoon in mouse brain slices (PFA fixed); can you recommend any one for this?
   • After searching through our product catalog list, we have found the following antibodies which may be of interest: Bassoo. Using the filters on the left column of the site should allow you to filter by species and desired application. Any antibody that is listed to show validation for use in IHC-P and has also been validated in mouse samples will be backed by our 100% Guarantee. Under the terms and conditions of the guarantee, Novus will provide you with a full refund or replacement should the antibody fail to perform, as specified, in samples expressing the protein.
2. We are interested in Anti Bassoon Ab (NBP1-46351), but we need the exact epitope sequence.
   • Unfortunately we cannot provide the specific immunogen sequence as it is proprietary; however I can tell you that the immunogen is found between amino acids 1000 and 1100 in the parental protein.

Bioinformatics

• Gene Symbol: BSN
• Entrez:
  • 8927(Human)