Bat Immunoglobulin Antibody (BT1-4F10) - BSA Free

Western Blot: Bat Immunoglobulin Antibody (BT1-4F10) [NBP2-23483] - Analysis of whole serum as detected by NBP2-23483. Left side shows the non-reduced lane, mainly IgG because all the chains are attached to each other. The right side is the reduced lane, where the different chains are separated and the one directly bound by the mAb (Light chain) is visualized.

• Reactivity: Bt
• Applications: WB, ELISA, Flow, ICC/IF, IP
• Clone: BT1-4F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Bat Immunoglobulin Antibody (BT1-4F10) - BSA Free Summary

• Immunogen: Microchiropteran bat (Eptesicus fuscus) spleen cells.
• Specificity: Immunoglobulin L (Light) chain.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunoprecipitation
  • Western Blot 0.5 ug/ml-1.0 ug/ml
• Application Notes: This Bat Immunoglobulin (BT1-4F10) antibody is useful for Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Flow Cytometry. This antibody is useful for detecting rabies in bats and identifies all immunoglobulin classes: IgG, IgD, IgE, IgA and IgM.

Reactivity Notes

Microchiropteran bats (confirmed against big brown, little brown, Red, Silverhair, Hoary bats) and porcine. Does not react with Megachiropteran bats (confirmed against P. poliocephalus, P. giganteus, P. rodricensis, R. aegyptiacus, P. vampyrus, E. helvum, P.pumilus, P. hypomelanus), human, mouse, rat, hamster, canine, feline, guinea pig, and bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Background

The anti-bat (BT1-4F10) monoclonal antibody is a unique antibody that is able to distinguish between rabies infected versus non-infected bats in an indirect immunofluorescence assay commonly used to detect rabies but never before in bats. One of the problems with studying bat immunology is that no serologic assay exists to probe bat responses to various pathogens such as rabies, severe acute respiratory syndrome (SARS) and Geomyces destructans, the etiologic agent of white nose syndrome, a condition associated with an unprecedented bat mortality event in the United States. This antibody is helping fill that gap. Emerging pathogens, many of them viruses, continue to surprise researchers by an underappreciated source, bats. Although bats are among the most abundant, diverse and geographically dispersed orders of terrestrial mammals, typically little has been studied about the bat as a host carrying microorganisms and transmitting pathogens to other species. In fact, bats are increasingly proving to be rich reservoirs of emerging viruses and re-emerging viruses as people disrupt by encroaching natural areas exposing not only ourselves, but our domesticated animals to viruses that earlier lacked opportunities to jump species.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bat Immunoglobulin Antibody (NBP2-23483)(2)

Publications using NBP2-23483

• Hall JS, Knowles S, Nashold SW et al. Experimental challenge of a North American bat species, big brown bat (Eptesicus fuscus), with SARS-CoV-2 Transboundary and emerging diseases 2020-12-09 [PMID: 33295095] (ELISA, Bat)
  
  Details:
  Eptesicus fuscus
• Irving AT, Rozario P, Kong PS et al. Robust dengue virus infection in bat cells and limited innate immune responses coupled with positive serology from bats in IndoMalaya and Australasia Cell. Mol. Life Sci. 2019-07-27 [PMID: 31352533]