Recombinant Human Batroxobin Protein

Product Discontinued

Recombinant Human Batroxobin Protein Summary

• Description: A biologically active protein to Batroxobin.
  
  A biologically active protein to Batroxobin.
  
  Amino Acid Sequence: VIGGDECDIN EHPFLAFMYY SPRYFCGMTL INQEWVLTAA HCNRRFMRIHLGKHAGSVAN YDEVVRYPKE KFICPNKKKN VITDKDIMLI RLDRPVKNSEHIAPLSLPSN PPSVGSVCRI MGWGAITTSE DTYPDVPHCA NINLFNNTVCREAYNGLPAK TLCAGVLQGG IDTCGGDSGG PLICNGQFQG ILSWGSDPCAEPRKPAFYTK VFDYLPWIQS IIAGNKTATC P
• Details of Functionality: Fully biologically active when compared to standard. The biologically activity of rRpBX is no less than 500KU/mg (Klobusitzky Unit). One KU is defined as the amount of enzyme which coagulates standard human plasma incubated at 37 C in vitro within 60 +/- 20 seconds.
• Protein/Peptide Type: Recombinant Protein
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.5EU/mg of rRpBX (Sneak) as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated sodium acetate buffer solution.
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Background

Batroxobin is serin protease secreted by the sneak venom gland. It can release fibrinopeptide A by cleaving between 16 Arg-17 Gly in the A (alpha)-chain of fibrinogen, and leading to the clot formation through aggregation of formed of fibrin I monomer or Des-AA-monomer. But the specificity of further degradation of fibrinogen varies with species origin of the enzyme. In contrast to thrombin, it does not affect the functions of platelets. Hence, BX is used for the investigation of the last phase of blood coagulation. Due to its heparin insensitivity it can detect fibrinogen polymerization disorders even in the presence of heparin. Additionally, it is available under the name Defibrase (Pentapharm) for the treatment of thrombotic diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.