Bax Antibody (6A7) - Azide and BSA Free

Western Blot: Bax Antibody (6A7) [NBP1-28566] - rOPN administration elevated the expression of autophagy-related proteins while suppressing apoptosis in rat brain at 24 h after SAH. The effects of rOPN on expression levels of Bax, mean +/- SD is 1.006 +/- 0.321 in Sham group, 37.47 +/- 10.86 in SAH + Vehicle group, 23.83 +/- 8.143 in SAH + rOPN group, F = 33.13, in the left hemisphere of rat brain at 24 h after SAH. Sample size is 18, n = 6 per group. Data were presented as mean +/- SD. *P < .05, ***P < .001 vs Sham group; #P < .05, ##P < .01 vs SAH + Vehicle group Image collected and cropped by CiteAb from the following publication (//onlinelibrary.wiley.com/doi/abs/10.1111/cns.13199) licensed under a CC-BY license.

Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566] - Mouse retina with no pretreatment. IHC-F image submitted by a verified customer review

Western Blot: Bax Antibody (6A7) [NBP1-28566] - Analysis of lysates from human metastatic pancreatic cancer cell line L3.6pl using Bax Antibody (clone 6A7; Lot # B168-P780E). This image was submitted via reviews by a verified end user.

Western Blot: Bax Antibody (6A7) [NBP1-28566] - Total cell lysates from NIH/3T3 cells were resolved by electrophoresis, transferred to PVDF membrane, and probled with Mouse Anti-Bax UNLB.

• Reactivity: Hu, Mu, Rt, Po, Bv, Pm
• Applications: WB, Func, IHC
• Clone: 6A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.1 mg/ml

Bax Antibody (6A7) - Azide and BSA Free Summary

• Immunogen: KLH-conjugated peptide corresponding to amino acids 12-24 located near the N-terminus common to human, mouse and rat Bax.
• Specificity: The monoclonal antibody 6A7 reacts with human, mouse and rat Bax but does not bind the soluble cytosolic form of Bax; however, treatment of cells with non-ionic detergents exposes the epitope and allows binding of 6A7 to monomeric forms of Bax but not Bax complexed with either Bcl-2 or Bcl-xL.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BAX
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Functional
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Western Blot < = 1 ug/ml
• Application Notes: Use in IHC-P reported in scientific literature (PMID:33143886) Use in functional reported in scientific literature (PMID:31410057). Bax antibody validated for IHC-F, WB from a verified customer reviews.

Reactivity Notes

Use in Porcine reported in scientific literature (PMID:34243771) Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Borate buffered saline, pH 8.2
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Bax Antibody (6A7) - Azide and BSA Free

• apoptosis regulator BAX
• Bax
• BCL2-associated X protein
• Bcl2-L-4
• BCL2L4bcl2-L-4
• Bcl-2-like protein 4

Background

Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems. (2) It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment. (3) Bax is a member of the Bcl-2 family of apoptosis-associated proteins and, although it is similar in structure to Bcl-2, Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and subsequent cell death in a caspase-independent manner, presumably via their ion channel-forming activity. These channels may then function to promote a mitochondrial permeability transition or to puncture the outer mitochondrial membrane. In addition to forming homodimer, Bax can heterodimerize with Bcl-2 and Bcl-XL proteins. This heterodimerization between pro- and anti-apoptotic family members may serve a titration function and act as a sensor for the programmed cell death program. (4-13) The monoclonal antibody 2D2 is specific for human Bax. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bax Antibody (NBP1-28566)(20)

Publications using NBP1-28566

• Yang G, Xiang J, Yang X et al. Nuclear translocation of SIRT4 mediates deacetylation of U2AF2 to modulate renal fibrosis through alternative splicing-mediated upregulation of CCN2 eLife 2024-11-04 [PMID: 39495216]
• Victorelli S, Salmonowicz H, Chapman J et al. Apoptotic stress causes mtDNA release during senescence and drives the SASP Nature 2023-10-01 [PMID: 37821702] (Co-IP, Mouse)
• Ghosh S, Singh R, Vanwinkle ZM et al. Microbial metabolite restricts 5-fluorouracil-resistant colonic tumor progression by sensitizing drug transporters via regulation of FOXO3-FOXM1 axis Theranostics 2022-07-18 [PMID: 35910798] (WB, Human)
  
  Details:
  Dilutions: 1:500
• Simbulan-Rosenthal CM, Haribabu Y, Vakili S et al. Employing CRISPR-Cas9 to Generate CD133 Synthetic Lethal Melanoma Stem Cells International journal of molecular sciences 2022-02-20 [PMID: 35216449] (WB, Human)
• Mehanna ET, Khalaf SS, Mesbah NM Et al. Anti-oxidant, anti-apoptotic, and mitochondrial regulatory effects of selenium nanoparticles against vancomycin induced nephrotoxicity in experimental rats Life sciences 2021-10-26 [PMID: 34715137] (WB, Rat)
• Hale BJ, Li Y, Adur MK et al. Characterization of the effects of heat stress on autophagy induction in the pig oocyte Reproductive biology and endocrinology : RB&E 2021-07-09 [PMID: 34243771] (IF/IHC)
• Song B, Wei D, Yin G et al. Critical role of SIRT1 upregulation on the protective effect of lncRNA ANRIL against hypoxia/reoxygenation injury in H9c2 cardiomyocytes Molecular medicine reports 2021-08-01 [PMID: 34080028] (WB, Rat)
• Gul HF, Ilhan N, Ilhan N et al. The Combined Effect of Pomegranate Extract and Tangeretin on the DMBA-induced Breast Cancer Model The Journal of nutritional biochemistry 2020-12-13 [PMID: 33326843] (WB, Rat)
• Arman T, Lynch KD, Goedken M, Clarke JD Sub-chronic microcystin-LR renal toxicity in rats fed a high fat/high cholesterol diet Chemosphere 2020-10-27 [PMID: 33143886] (IHC-P, Rat)
• Sun F, Du J, Li H et al. FABP4 inhibitor BMS309403 protects against hypoxia-induced H9c2 cardiomyocyte apoptosis through attenuating endoplasmic reticulum stress J. Cell. Mol. Med. 2020-09-07 [PMID: 32896039] (WB, Rat)
• Tang H, Gamdzyk M, Huang L et al. Delayed recanalization after MCAO ameliorates ischemic stroke by inhibiting apoptosis via HGF/c-Met/STAT3/Bcl-2 pathway in rats Exp. Neurol. 2020-05-16 [PMID: 32428505] (WB, Rat)
• Sun CM, Enkhjargal B, Reis C et al. Osteopontin attenuates early brain injury through regulating autophagy-apoptosis interaction after subarachnoid hemorrhage in rats CNS Neurosci Ther 2019-08-22 [PMID: 31436915] (WB, Rat)
• Gong X, Xu B, Zi L, Chen X, miR-625 reverses multidrug resistance in gastric cancer cells by directly targeting ALDH1A1 CMAR 2019-07-01 [PMID: 31410057] (Func, Human)
• Zheng W, Matei N, Pang J et al. Delayed recanalization at 3 days after permanent MCAO attenuates neuronal apoptosis through FGF21/FGFR1/PI3K/Caspase-3 pathway in rats Exp. Neurol. 2019-07-08 [PMID: 31295445] (WB, Rat)
• Yu, CC;Li, CF;Chen, IH;Lai, MT;Lin, ZJ;Korla, PK;Chai, CY;Ko, G;Chen, CM;Hwang, T;Lee, SC;Sheu, JJ; YWHAZ amplification/overexpression defines aggressive bladder cancer and contributes to chemo-/radio-resistance by suppressing caspase-mediated apoptosis J. Pathol. 2019-04-03 [PMID: 30945298] (WB, Human)
• Zhou X, Liu S, Lin X et al. Metformin Inhibit Lung Cancer Cell Growth and Invasion in Vitro as Well as Tumor Formation in Vivo Partially by Activating PP2A Med. Sci. Monit. 2019-01-29 [PMID: 30693913] (WB, Human)
• Xu D, Chen P, Wang B et al. NIX-mediated mitophagy protects against proteinuria-induced tubular cell apoptosis and renal injury. Am. J. Physiol. Renal Physiol. 2018-09-12 [PMID: 30207166] (WB, Rat)
• Xie Z, Enkhjargal B, Wu L et al. Exendin-4 attenuates neuronal death via GLP-1R/PI3K/Akt pathway in early brain injury after subarachnoid hemorrhage in rats. Neuropharmacology. 2017-10-04 [PMID: 28986282] (WB, Rat)
• Shi X, Xu L, Doycheva DM et al. Sestrin2, as a negative feedback regulator of mTOR, provides neuroprotection by activation AMPK phosphorylation in neonatal hypoxic-ischemic encephalopathy in rat pups J. Cereb. Blood Flow Metab. 2016-07-05 [PMID: 27381825]
• Barreto-Vianna AR, Aguila MB, Mandarim-de-Lacerda CA. Effects of liraglutide in hypothalamic arcuate nucleus of obese mice Obesity (Silver Spring) 2016-03-01 [PMID: 26916241] (WB)

Reviews for Bax Antibody (NBP1-28566) (4)

Average Rating: 4.8

(Based on 4 reviews)

We have 4 reviews tested in 3 species: Human, Mouse, Human and Mouse.

reviewed by: Sweta Ghosh WB Human and Mouse 04/19/2024

Summary

• Application: Western Blot
• Sample Tested: Cell lysate from colon cancer cell line,Melanoma cells
• Species: Human and Mouse
• Lot: H1815-M531

reviewed by: Hayk Simonyan WB Mouse 06/23/2017

Summary

• Application: Western Blot
• Sample Tested: mouse liver
• Species: Mouse

reviewed by: Verified Customer WB Human 03/31/2014

Summary

• Application: Western Blot
• Species: Human
• Lot: B168-P780E

reviewed by: Verified Customer IHC-Fr Mouse 07/28/2012

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: mouse retina
• Species: Mouse
• Lot: B168-S1412

Bioinformatics

• Gene Symbol: BAX
• Entrez:
  • 581(Human)
  • 12028(Mouse)
  • 24887(Rat)
• Uniprot:
  • Q07812()