Bax Antibody (SP47)

Product Discontinued

Bax Antibody (SP47) Summary

• Immunogen: Synthetic peptide corresponding to internatl region of human Bax region
• Localization: Cytoplasm, Membrane
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BAX
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: This product is useful for Immunohistochemistry Paraffin. Antigen retrieval can be performed using a Citrate process.

Reactivity Notes

Human. Predicted cross-reactivity based on sequence identity: Bovine, Mouse, Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.6) and 1.0% BSA
• Preservative: Sodium Azide
• Purity: Protein A or G purified

Notes

For a positive control use Breast carcinoma.

Alternate Names for Bax Antibody (SP47)

• apoptosis regulator BAX
• Bax
• BCL2-associated X protein
• Bcl2-L-4
• BCL2L4bcl2-L-4
• Bcl-2-like protein 4

Background

Bax is a 21 kD pro-apoptotic protien known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, and nucleus and is highly expressed in hematopoietic stem cells, the ovary, and in the lymph node. Bax binds the anti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins such as Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptotic stimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome c to pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionally activate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers. The Poly6251 antibody has been reported to be useful for Western blotting, immunoprecipitation, and immunohistochemistry of human, mouse, and rat Bax protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Bax Antibody (NBP1-49811). (Showing 1 - 1 of 1 FAQ).

1. Could you inform us the concentration of sodium azide for Bax Antibody (SP47) (#NBP1-49811)?
   • NBP1-49811 contains less than 0.1% Sodium Azide.

Bioinformatics

• Gene Symbol: BAX
• Entrez:
  • 280730(Bovine)
  • 581(Human)
  • 12028(Mouse)
  • 24887(Rat)
• Uniprot:
  • Q07812()