beta-1 Adrenergic R/ADRB1 Antibody

Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NBP2-39091] - Analysis in human heart muscle and skin tissues using NBP2-39091 antibody. Corresponding ADRB1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NBP2-39091] - Staining of human fallopian tube shows no positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NBP2-39091] - Staining of human heart muscle shows moderate membranous positivity in cardiomyocytes.

Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NBP2-39091] - Staining of human lymph node shows no positivity in germinal center cells as expected.

Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NBP2-39091] - Staining of human skin shows no positivity in squamous epithelial cells as expected.

Product Discontinued

beta-1 Adrenergic R/ADRB1 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: ASLLPPASESPEPLSQQWTAGMGLL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADRB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for beta-1 Adrenergic R/ADRB1 Antibody

• ADRB1
• ADRB1R
• ADRB1RRHR
• adrenergic, beta-1-, receptor
• B1AR
• beta-1 Adrenergic R
• beta-1 adrenergic receptor
• beta1 AdrenergicR
• beta-1 AdrenergicR
• Beta-1 adrenoceptor
• Beta-1 adrenoreceptor
• BETA1AR

Background

Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for beta-1 Adrenergic R/ADRB1 Antibody (NBP2-39091) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rhesus Macaque.

reviewed by: Verified Customer IHC-Fr Rhesus Macaque 12/16/2022

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Fixed frozen rhesus macaque brain sections,Rhesus macaque fixed brain tissue
• Species: Rhesus Macaque
• Lot: 000025654

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.*** Protocol: 1). Fixation was exactly as described in: Gray, Daniel T et al. “Retrosplenial cortex microglia and perineuronal net densities are associated with memory impairment in aged rhesus macaques.” Cerebral cortex (New York, N.Y. : 1991), bhac366. 28 Sep. 2022, doi:10.1093/cercor/bhac366 2). Day1: The 30μm fixed section of brain tissue was washed in 0.1M Tris Buffer solution (TBS); for 3 timesx5min. It then underwent an antigen retrieval procedure in a sodium citrate buffer solution (pH 6); for 30 minutes at 80-85°C. Following antigen retrieval, the tissue was washed again in TBS (3timesx5min), then incubated for 1 hour in a blocking solution of TBS containing 3% Normal Donkey Serum and 0.03% TritonX-100. The section was then incubated in the rabbit anti-Beta1 antibody (1:100); in 1ml of blocking solution overnight at room temperature. 3). Day 2: The section was washed with TBS (4 timex10min) then incubated for 2 hours at room temperature with a secondary antibody, anti-rabbit Alexa647 F(ab’)2 Fragment (1:500) diluted in 1ml of blocking solution. Tissue was washed 4 timesx10 min in TBS and then mounted with 80% glycerol. Sections were imaged using a Zeiss LSM880 confocal microscope in lambda mode, and images underwent linear unmixing procedures to remove lipofuscin and separate channels as described in the above publication.

Bioinformatics

• Gene Symbol: ADRB1
• Uniprot:
  • P08588()