beta-Catenin Antibody

Western Blot: beta-Catenin Antibody [NB100-2141] - MDBK cells were separated on SDS-PAGE and probed with anti-beta Catenin.

Product Discontinued

beta-Catenin Antibody Summary

• Immunogen: Synthetic peptide: PGDSNQLAWFDTDL conjugated to KLH, corresponding to amino acids 768-781 of Human or mouse, Catenin.
• Epitope: a.a. 768-781
• Localization: Cytoplasmic (high level of ser/thr phosphorylation) or bound to CDH1. Translocates to the nucleus (low level of ser/thr phosphorylation).
• Marker: Epithelial Cell Marker, Adherens Junction Marker
• Specificity: Reacts in dot blot with beta-catenin peptide 768-781 conjugated to BSA. In immunoblots, reacts with a 94kD protein in extracts of Madin-Darby Bovine Kidney (MDBK) cultured cells. Specific staining is inhibited following pre-incubation of the antiserum with immunizing peptide. The antiserum has been treated to remove lipoproteins. The antibody does not cross react with a-catenin or y-catenin (plakoglobin).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTNNB1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:2000
  • Western Blot 1:4000
• Application Notes: Western blot, Dot blot, IHC-Fr. Use at an assay dependant dilution. IHC-Fr: Use bovine kidney frozen sections or cultured MDBK cells. WB: MDBK cells extract. Optimal dilutions/concentrations should be determined by the end user. Although not tested, this antibody may be useful in Immunocytochemistry.
• Theoretical MW: 94 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  beta-Catenin Overexpression Lysate

Reactivity Notes

Cross-reacts with Zebrafish. Expected to cross-react with a wide range of species due to sequence identity. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for beta-Catenin Antibody

• bCatenin
• beta 1 (88kD)
• beta-Catenin
• catenin (cadherin-associated protein), beta 1, 88kDa
• catenin beta-1
• CTNNB
• CTNNB1
• DKFZp686D02253
• FLJ25606
• FLJ37923

Background

The distinct peripheral cytosolic proteins, a-, b- and g-catenin (102 kDa, 94 kDa and 86 kDa respectively), are found in varying abundance in many developing and adult tissues.1,2,3 The catenins bind, directly or indirectly, to the conserved cytoplasmic tail domain of the celladhesion cadherins. Cadherins are transmembrane cell surface glycoprotein molecules, concentrated at adherens junctions that mediate calcium-dependent intercellular interactions and are important for tissue morphogenesis.4 The linkage of the epithelial Ecadherin/ uvomorulin to actin is essential for the cell binding function of this cadherin. Catenins also link E cadherin to other integral membrane proteins such as Na+/K+-ATPase, or to cytoplasmic proteins such as fodrin, ankyrin, Src and Yes kinases5 and are modulated by Wnt-1 protooncogene.6,7 They are considered good candidates for mediating transduction of cell-cell contact positional signals to the cell interior.4,5 Within its conserved regions a-catenin shows 30% identity to vinculin, a protein found mainly in focal cell-cell and cell substrate adhesions.2,3 Vinculin is known to interact with a-actinin, which in turn is associated with actin filaments in their site of attachment to the cell membrane focal contacts. The protein, a-catenin, is capable of interacting with N-cadherin and P-cadherin. Absence of a-catenin is found in certain tumor cell lines.8 Frequent reduction of a-catenin levels in human carcinomas of the esophagus, stomach and colon is reported9 Enhancement of tumor cell invasion and metastatic ability of such cells following catenins down-regulation is speculated. Prostate cancer development appears to be correlated with a-catenin gene deletions. Plakoglobin (probably identical to g-catenin) and b-catenin are structural and possibly functional mammalian homologues of armadillo (arm), a Drosophila protein involved in signal transduction. The protein, b-catenin, binds directly to the cytoplasmic tail of Ecadherin. It binds to the amino terminus of a-catenin and also interacts with the cytosolic protein product of the human tumor suppressor gene APC.10 Mutations in this gene occur early in colon carcinogenesis. Such mutations are linked to familial adenomatous polyposis and to progression of sporadic colorectal and gastric tumors. The preferential interaction of b-catenin with the APC protein involves a 15-amino acid repeat in the latter11 and b-catenin cell levels seem to be controlled by APC.12 The central core region of b-catenin is involved in mediation of the interaction of cadherin-catenin complex with the epidermal growth factor receptor.13 The protein, b-catenin, is the target of two signal transduction pathways mediated by the protooncogenes Src and Wnt-1. p120cas which exhibits structural similarity to b-catenin and plakoglobin may represent another catenin associated with cadherin.14 Polyclonal antibodies against defined type-specific catenin peptides are useful tools for the study of these proteins. Such antibodies recognize the respective catenin type in a variety of immunological techniques such as immunoprecipitation, immunoblotting, immunofluorescence and immunoperoxidase in different cell types from various species.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Catenin Antibody (NB100-2141)(3)

Publications using NB100-2141

• Dalmasso AP, Goldish D, Benson BA et al. IL-4 Induces Up-regulation of Endothelial Cell Claudin-5 through Activation of FoxO1: Role in Protection from Complement-mediated Injury. J Biol Chem. 2013-11-26 [PMID: 24280217] (WB, Porcine)
• Hsieh YT, Chou MM, Chen HC, Tseng JJ. IMP1 promotes choriocarcinoma cell migration and invasion through the novel effectors RSK2 and PPME1. Gynecol Oncol. 2013-10-01 [PMID: 23911878]
• Goentoro L, Kirschner MW. Evidence that fold-change, and not absolute level, of beta-catenin dictates Wnt signaling. Mol Cell 2009-12-11 [PMID: 20005849]

Bioinformatics

• Gene Symbol: CTNNB1
• Entrez:
  • 1499(Human)
  • 12387(Mouse)
  • 84353(Rat)