beta-Catenin [p Ser33, p Ser37] Antibody

Product Discontinued

beta-Catenin [p Ser33, p Ser37] Antibody Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser33/37 conjugated to KLH
• Modification: p Ser33, p Ser37
• Localization: Cytoplasmic (high level of ser/thr phosphorylation) or bound to CDH1. Translocates to the nucleus (low level of ser/thr phosphorylation).
• Marker: Epithelial Cell Marker, Adherens Junction Marker
• Specificity: Specific for the ~83k beta-catenin phosphorylated at Ser33 and Ser37.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTNNB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Control:

  beta-Catenin Overexpression Lysate

Reactivity Notes

Reactivity assumed based on 100% sequence identity: Mouse, Rat and Xenopus.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for beta-Catenin [p Ser33, p Ser37] Antibody

• bCatenin
• beta 1 (88kD)
• beta-Catenin
• catenin (cadherin-associated protein), beta 1, 88kDa
• catenin beta-1
• CTNNB
• CTNNB1
• DKFZp686D02253
• FLJ25606
• FLJ37923

Background

B-Catenin is a central component of the cadherin cell adhesion complex and plays an essential role in neural development in the Wingless/Wnt signaling pathway (Ding and Dale, 2002; Polakis, 2000). The role of B-catenin in these processes is thought regulated by the sequential phosphorylation of Ser29 Ser33, Ser37 and Thr41 by glycogen synthase kinase 3B (GSK3B) (Liu et al., 2002). This hyperphosphorylation promotes the ubiquitylation and targeted destruction of B-catenin. Mutations in components of this phosphorylation regulated process that prevent B-catenin hyperphosphorylation by GSK3B are strongly associated with cancers (Wang et al., 2003; Polakis, 2000; Liu et al., 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Catenin Antibody (NB300-272)(4)

Publications using NB300-272

• Wang ZH, Vogelstein B, Kinzler KW. Phosphorylation of B-catenin at S33, S37, or T41 can occur in the absence of phosphorylation at T45 in colon cancer cells. Cancer Res 63:5234-5235. 2003-01-01 [PMID: 14500351]
• Ding Y, Dale, T. Wnt signal transduction: kinase cogs in a nano-machine. Trends Biochem Sci 27:327-329. 2002-01-01 [PMID: 12114015]
• Liu C, Li Y, Semenov M, Han C, Baeg GH, Tan Y, Zhang Z, Lin X, He X. Control of B-catenin phosphorylation/degradation by a dual-kinase mechanism. Cell 108:837-847. 2002-01-01 [PMID: 11955436]
• Polakis P. Wnt signaling cancer. Genes Dev 14:1837-1851. 2000-01-01 [PMID: 10921899]

Bioinformatics

• Gene Symbol: CTNNB1
• Entrez:
  • 1499(Human)
  • 84353(Rat)
• Uniprot:
  • P35222()