beta Galactosidase Activity Assay Kit (Colorimetric) Summary
| Description |
Detection instrument: Microplate reader (390 - 410 nm, optimum wavelength: 400 nm)
Assay time: 70 min |
| Standard Curve Range |
16.19 - 1200 U/L |
| Sensitivity |
16.19 U/L |
| Inter-Assay |
%CV < 6 |
| Intra-Assay |
%CV < 3 |
| Spike Recovery |
Average recovery rate: 101% |
| Kit Type |
Activity Assay Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- Microplate
- Plate Sealer
- Reagent 1: Buffer Solution
- Reagent 2: Substrate
- Reagent 3: Activator Agent
- Reagent 4: Chromogenic Agent
- Reagent 5: 20 mmol/L Standard Solution
|
Alternate Names for beta Galactosidase Activity Assay Kit (Colorimetric)
Background
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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Blogs on beta Galactosidase
![beta Galactosidase Activity Assay Kit (Colorimetric) [NBP3-24551] - Standard Reference Curve](http://images.novusbio.com/fullsize/nbp3-24551_beta-galactosidase-activity-assay-kit-colorimetric-2611202315565849.png "beta Galactosidase Activity Assay Kit (Colorimetric) [NBP3-24551] - Standard Reference Curve")
![beta Galactosidase Activity Assay Kit (Colorimetric) [NBP3-24551] - Assay Example](http://images.novusbio.com/fullsize/nbp3-24551_beta-galactosidase-activity-assay-kit-colorimetric-81202410583841.png "beta Galactosidase Activity Assay Kit (Colorimetric) [NBP3-24551] - Assay Example")
![Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis of a band at 117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1 ug of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green).](https://images.novusbio.com/images/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0014.jpg "Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis of a band at 117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1 ug of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green).")