| Reactivity | BaSpecies Glossary |
| Applications | Func |
| Concentration | LYOPH |
| Description | Native Escherichia coli beta galactosidase. Source:E. coli Amino Acid Sequence:UniProt P00722 |
| Details of Functionality | One unit hydrolyses 1 umole of p-nitrophenol-b-D-galactoside to p-nitrophenol per minute at 37C. |
| Source | E. coli |
| Protein/Peptide Type | Native Protein |
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| Theoretical MW | 540 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. After reconstitution store at -20C. |
| Buffer | PBS, Contains Sucrose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Reconstitution Instructions | Reconstitute with 1.0 ml sterile distilled water or aqueous buffer of choice. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix vial after reconstitution. |
| Publication using NBP2-62407 | Applications | Species |
|---|---|---|
| Lin W, Wang J, Ma I et al. Highly sensitive b-galactosidase detection using streptavidin-display E. coli and lateral flow immunoassay Sensors and Actuators A: Physical 2022-12-01 |
Research Areas for beta Galactosidase Native Protein (NBP2-62407)Find related products by research area.
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![Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis of a band at 117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1 ug of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green).](https://images.novusbio.com/images/beta-Galactosidase-Antibody-Western-Blot-NB600-305-img0014.jpg "Western Blot: beta Galactosidase Antibody [NB600-305] - Analysis of a band at 117 kDa (lane 1) corresponding to b-Gal present in a partially purified preparation (arrowhead). Approximately 1 ug of protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4 C followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 for 45 min at room temperature (800 nm channel, green).")