Reactivity | AllSpecies Glossary |
Applications | WB, Flow, Func, ICC/IF, IHC |
Clone | BTN/8672R |
Clonality | Monoclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Format | Azide and BSA Free |
Concentration | 1 mg/ml |
Description | Positive Controls: Biotinylated proteins in solution or on tissues Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required. |
Additional Information | Recombinant Monoclonal Antibody |
Immunogen | Biotinylated sheep immunoglobulin |
Localization | Depends on the target |
Specificity | This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds It has a very high affinity for biotin and is excellent for use in various amplification techniques In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining Anti-biotin antibody may be substituted to decrease this noise |
Isotype | IgG Kappa |
Clonality | Monoclonal |
Host | Rabbit |
Purity | Protein A purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
Buffer | 10mM PBS |
Preservative | No Preservative |
Concentration | 1 mg/ml |
Purity | Protein A purified |
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