Biotin Antibody (BTN/8672R) - Azide and BSA Free

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Formalin-fixed, paraffin-embedded human kidney stained with Biotin antibody (BTN/8672R).HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity AllSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IHC
Clone
BTN/8672R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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Biotin Antibody (BTN/8672R) - Azide and BSA Free Summary

Description
Positive Controls: Biotinylated proteins in solution or on tissues

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds It has a very high affinity for biotin and is excellent for use in various amplification techniques In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining Anti-biotin antibody may be substituted to decrease this noise
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • In-situ Hybridization
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Biotin Antibody (BTN/8672R) - Azide and BSA Free

  • Biotin
  • Vitamin B4

Background

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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