Biotin Antibody (SPM375)

Immunohistochemistry-Paraffin: Biotin Antibody (SPM375) [NBP1-46960] - Formalin-fixed, paraffin-embedded tonsil stained with Biotinylated Lambda Light Chain probe followed by anti-Biotin Monoclonal Antibody (SPM375).

Product Discontinued

Biotin Antibody (SPM375) Summary

• Immunogen: Biotinylated sheep immunoglobulin
• Localization: Depends on the target
• Specificity: It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 1-2ug/ml
  • Immunohistochemistry 1-2ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • In-situ Hybridization
  • Western Blot
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 244 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for Biotin Antibody (SPM375)

• Biotin
• Vitamin B4

Background

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.