Biotin Antibody [FITC] - F(ab)

Dot Blot results of Goat Biotin Antibody [FITC] - F(ab). Dots top row: Biotin-BSA Conjugate. Dots bottom row: BSA alone. Load: (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Goat Fab Anti-Biotin Antibody FITC at 1ug/mL in 1hr RT. Imaged with BioRad ChemiDoc, Fluorescein filter.

• Reactivity: All-NA
• Applications: WB, DB, ICC/IF, IHC, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: FITC
• Concentration: LYOPH

Biotin Antibody [FITC] - F(ab) Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)-Immunoglobulin and Protease free
  Color: Green Excitation Wavelength: 495 Emission Wavelength: 528
  Conjugation Chemistry: Isothiocyanate
  
  Fab fragment anti-Biotin Antibody [FITC]was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein
• Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Immunocytochemistry/ Immunofluorescence 1:500-1:2500
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:20-1:50
  • Knockout Validated
  • Western Blot 1:100-1:500
• Application Notes: This product has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Specific conditions for reactivity should be optimized by the end user.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water (or equivalent)

Alternate Names for Biotin Antibody [FITC] - F(ab)

• Biotin
• Vitamin B4

Background

Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.