![Antibody Labeling Kit [4001-0030] - When the biotinylated antibody is mixed with the streptavidin-gold conjugate it forms a complex that flows along the nitrocellulose membrane. Upon binding to the Protein A and Protein G concentrated on the Test line, a visible red line appears on the strip.](http://images.novusbio.com/fullsize/Antibody-Labeling-Kit-4001-0030-img0001.jpg "Antibody Labeling Kit [4001-0030] - When the biotinylated antibody is mixed with the streptavidin-gold conjugate it forms a complex that flows along the nitrocellulose membrane. Upon binding to the Protein A and Protein G concentrated on the Test line, a visible red line appears on the strip.")
| Description | Biotin Check&Go! allows scientists to confirm the success of their antibody biotinylation in one easy step. Unlike traditional methods of assessing the success of biotinylation, such as the HABA assay, which can be tedious and time consuming, Biotin Check&Go! is a simple immunochromatography test that requires only 10 minutes of your time and very small quantities of your antibody conjugate. The key components of the kit include a nitrocellulose membrane containing a Test line of immobilized Protein A and Protein G, both which have a high affinity for the Fc region of a variety of IgG molecules, and a 40nm InnovaCoat GOLD Streptavidin conjugate for detection.
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| Kit Type | Control Kit |
| Application Notes | This product is suitable for use with Lightning-Link Biotin antibody labeling kits as well as other biotinylation technologies. |
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| Storage | Storage of components varies. See protocol for specific instructions. |
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| Publication using 4001-0030 | Applications | Species |
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| Sharma P, Diergaarde B, Ferrone S et al. Melanoma cell-derived exosomes in plasma of melanoma patients suppress functions of immune effector cells Sci Rep 2020-01-09 [PMID: 31919420] |
