Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/403) [Biotin]

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Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, Flow, Func, ICC/IF, IHC
Clone
BTN/403
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Order Details

Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/403) [Biotin] Summary

Immunogen
Biotinylated sheep immunoglobulin
Localization
Depends on the target
Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • In-situ Hybridization
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/403) [Biotin]

  • 58-85-5
  • coenzyme R
  • d-biotin
  • Vitamin B7
  • vitamin H

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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