![Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8) - Azide and BSA Free [NBP2-34646] - Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain probe followed by anti-biotin MAb (Hyb-8). Note cell membrane & cytoplasmic staining.](http://images.novusbio.com/fullsize/Biotin-Vitamin-B7-or-Vitamin-H-Antibody-Hyb-8-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34646-img0001.jpg "Immunohistochemistry-Paraffin: Biotin (Vitamin B7 or Vitamin H) Antibody (Hyb-8) - Azide and BSA Free [NBP2-34646] - Formalin-fixed, paraffin-embedded human tonsil stained with biotinylated Lambda Light Chain probe followed by anti-biotin MAb (Hyb-8). Note cell membrane & cytoplasmic staining.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, Flow, Func, ICC/IF, IHC |
| Clone | Hyb-8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32811). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Biotinylated sheep immunoglobulin |
| Localization | Depends on the target |
| Specificity | It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This monoclonal antibody is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 244 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")