Western Blot: BirA Antibody (6C4c7) [NBP2-59938] - Total cell lysate of parental human fibroblasts (HF) (lane 1) or HF stably expressing GFP-NLS-BirA without (lane 2) or with induced expression (lane 3) were transferred ...read more
Immunocytochemistry/ Immunofluorescence: BirA Antibody (6C4c7) [NBP2-59938] - HeLa cells stably expressing a BirA tagged TorsinAE171Q were fixed in (A) 4% PFA or (B) cold methanol and stained with anti-BirA 6C4c7 ...read more
Yeast reactivity reported in scientific literature (PMID:32877643).
Packaging, Storage & Formulations
Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified
Alternate Names for BirA Antibody (6C4c7) - BSA Free
Bifunctional protein birA
Bio R
bioR
Biotin [acetyl CoA carboxylase] synthetase
BirA
dhbB
ECK3965
Background
BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for BirA Antibody (NBP2-59938)(4)
We have publications tested in 2 confirmed species: Human, Yeast.
We have publications tested in 2 applications: ICC/IF, WB.
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![Western Blot: BirA Antibody (6C4c7) [NBP2-59938] - Total cell lysate of parental human fibroblasts (HF) (lane 1) or HF stably expressing GFP-NLS-BirA without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BirA with NBP2-59938. The predicted GFP-NLS-BirA molecular weight is 66kDa.](http://images.novusbio.com/fullsize/antibody/nbp2-59938_mouse-bira-mab-6c4c7-bsa-free-western-blot-38202313527..jpg "Western Blot: BirA Antibody (6C4c7) [NBP2-59938] - Total cell lysate of parental human fibroblasts (HF) (lane 1) or HF stably expressing GFP-NLS-BirA without (lane 2) or with induced expression (lane 3) were transferred to a nitrocellulose membrane and blotted for BirA with NBP2-59938. The predicted GFP-NLS-BirA molecular weight is 66kDa.")
![Immunocytochemistry/Immunofluorescence: BirA Antibody (6C4c7) [NBP2-59938] - HeLa cells stably expressing a BirA tagged TorsinAE171Q were fixed in (A) 4% PFA or (B) cold methanol and stained with anti-BirA 6C4c7 monoclonal antibody (green). The nuclei were counter-stained with Hoechst.](http://images.novusbio.com/fullsize/antibody/nbp2-59938_mouse-bira-mab-6c4c7-bsa-free-immunocytochemistry-immunofluorescence-38202313619..jpg "Immunocytochemistry/Immunofluorescence: BirA Antibody (6C4c7) [NBP2-59938] - HeLa cells stably expressing a BirA tagged TorsinAE171Q were fixed in (A) 4% PFA or (B) cold methanol and stained with anti-BirA 6C4c7 monoclonal antibody (green). The nuclei were counter-stained with Hoechst.")