Recombinant Human BMP-7 Protein

SDS-Page: Human BMP-7 Protein [NBP2-34933]

• Reactivity: Hu
• Applications: WB, ELISA, PAGE
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human BMP-7 Protein Summary

• Description: A monomeric, non-glycosylated polypeptide chain containing 139 amino acids corresponding to BMP-7 Source: E. coli

  Uniprot ID: P18075

  Amino Acid Sequence: STGSKQRSQN RSKTPKNQEA LRMANVAENS SSDQRQACKK HELYVSFRDL GWQDWIIAPE GYAAYYCEGE CAFPLNSYMN ATNHAIVQTL VHFINPETVP KPCCAPTQLN AISVLYFDDS SNVILKKYRN MVVRACGCH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: BMP7
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of BMP-7 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA
  • SDS-Page
  • Western Blot
• Application Notes: 1. Molecular standard (Western, ELISA) in studying secreted BMP-7;
  2. Preparing antibodies for BMP-7 monomer;
  3. Molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE.
• Theoretical MW: 15.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human BMP-7 Protein

• BMP7
• BMP-7
• bone morphogenetic protein 7
• Eptotermin alfa
• OP-1
• OP-1OP1
• Osteogenic protein 1

Background

Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-b superfamily member. Human BMP-7 is synthesized with a propeptide and a growth factor domain. It is secreted as a tetramer that consists of two cleaved propeptides and two disulfide-linked growth factor domains. Mature BMP-7 can also form disulfide-linked heterodimers with BMP-2 or BMP-4. Secreted BMP-7 is immobilized in the ECM through interactions with fibrillin. BMP-7 signals through complexes of type 2 receptors (Activin RIIA, Activin RIIB, and BMPR-II) and type 1 receptors (Activin RIA, BMPR-IA, and BMPR-IB). BMP-7 promotes new bone formation and nephron development, inhibits the branching of prostate epithelium, and antagonizes epithelial-mesenchymal transition (EMT). In disease, BMP-7 inhibits tumor growth and metastasis, ameliorates fibrotic damage in nephritis, and promotes neuroregeneration following brain ischemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: BMP7
• Uniprot:
  • P18075()
  • P18075()
  • P18075()