BNIP3 Antibody (ANa40) [DyLight 350]

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: ANa40
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 350

BNIP3 Antibody (ANa40) [DyLight 350] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Recombinant fragment (Residues 1-163 of Human BNIP3).
• Epitope: The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.
• Localization: Mitochondrial and Nuclear membrane
• Specificity: BNIP3 (ANa40)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BNIP3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for BNIP3 Antibody (ANa40) [DyLight 350]

• BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• BCL2/adenovirus E1B 19kDa interacting protein 3
• BCL2/adenovirus E1B 19kD-interacting protein 3
• BNIP3
• Nip3

Background

BCL2/adenovirus E1B 19kD protein-interacting protein 3 (BNIP3) is a mitochondrial protein critical for apoptotic events in response to adenovirus infection. It has been implicated in causing a necrotic-like cell death pattern in cells, induced by CD47. This effect has been seen in many cancer cells, and its mis-regulation causes tumor cells to be impervious to normal autophagic events. The tumor suppressor p53 represses the expression of BNIP3, often as a consequence of hypoxic micro-environments as seen in tumors. When BNIP3 is correctly expressed and activated, it translocates to the mitochondria and instigates apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BNIP3