BOK Antibody [Unconjugated]

Western blot shows lysates ofE. colitransfected with an inducible expression vector containing rat BOK. PVDF membrane was probed with 0.8 µg/mL Rabbit Anti-Rat BOK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF825) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for BOK was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

• Reactivity: Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

BOK Antibody [Unconjugated] Summary

• Immunogen: KLH-coupled rat BOK synthetic peptide
  ARLLRAGLSWSAPERASPAPGC
• Specificity: Detects rat BOK in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BOK
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.8 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BOK Antibody [Unconjugated]

• bcl2-L-9
• BCL2L9bcl-2-related ovarian killer protein
• Bcl-2-like protein 9
• BCL2-related ovarian killer
• BOK
• BOKL
• hBOK
• MGC4631

Background

BOK (Bcl-2-related Ovarian Killer) is a 23-kDa pro-apoptotic member of the Bcl-2 family of proteins. BOK has three conserved Bcl-2 homology domains (BH1, 2, and 3) and a C-terminal transmembrane domain, but does not contain the anti-apoptotic BH4 domain. BOK interacts strongly with some anti-apoptotic Bcl-2 proteins (Mcl-1, BHRF1, and A1) and not at all with pro-apoptotic Bcl-2 proteins. The tissue distribution of BOK is restricted to reproductive tissues and it is highly expressed in ovary, testis, and uterus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BOK