Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1))

Product Discontinued

Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1)) Summary

• Immunogen: Made to Chemical BrdU
• Localization: Nuclear
• Marker: Proliferation Marker
• Specificity: This reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. It detects nucleated cells in S-Phase which have had BrdU incorporated into their DNA. Also reacts weakly with chlorodeoxyuridine, but does not cross-react with thymidine or iododeoxyuridine.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Cell Proliferation
  • Flow Cytometry 1:25-1:200
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:25-1:200
  • Western Blot
• Application Notes: Clone BU1/75 (ICR1) can be used for labeling paraffin-embedded tissue sections fixed in formalin. Denaturation of the DNA is critical for successful staining of BrdU. This can be achieved by exposing cells to heat, or acid. For heat-induced epitope retrieval, 10mM citrate buffer pH6.0 is recommended. Alternatively, a 30 min incubation in 2M HCl can be performed. The HCl must then be neutralized for 2 min with 0.1 M Na2B4O7. Pretreatment of tissues with proteinase K should be avoided. IHC-F was reported in scientific literature. Use in WB reported in scientific literature (PMID 27425845). Use in Cell Proliferation reported in scientific literature (PMID:34440835).

Reactivity Notes

Monkey reactivity reported in (PMID: 28566378). Rat reactivity reported in (PMID:23966683). Drosophila reactivity reported in (PMID:22438819).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Bromodeoxyuridine/BrdU Antibody (BU1/75 (ICR1))

• 5-bromo-2-deoxyuridine
• BrdU
• Bromodeoxyuridine
• BUdr

Background

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bromodeoxyuridine/BrdU Antibody (NB500-169)(145)

Publications using NB500-169

• Bu F, Min J, Razzaque M et al. Activation of cerebral Ras-related C3 botulinum toxin substrate (Rac) 1 promotes post-ischemic stroke functional recovery in aged mice Neural Regeneration Research 2024-04-01 [PMID: 37843224] (IHC, Mouse)
  
  Details:
  1:500 dilution
• Gupta N, Huang TT, Nair JR et al. BLM overexpression as a predictive biomarker for CHK1 inhibitor response in PARP inhibitor-resistant BRCA-mutant ovarian cancer Science translational medicine 2023-06-21 [PMID: 37343085]
• Wang YC, Kelso AA, Karamafrooz A et al. Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination bioRxiv 2023-02-03 [PMID: 36778247] (Human)
• van Schie J, de Lint K, Pai G et al. MMS22L-TONSL functions in sister chromatid cohesion in a pathway parallel to DSCC1-RFC Life Science Alliance 2023-02-01 [PMID: 36622344] (Immunocytochemistry/ Immunofluorescence, Human)
• Bass RG, Husain Z, Dahora LI, Thompson C Equilin does not affect thyroid hormone signaling in the developing Xenopus laevis tadpole brain bioRxiv
• Sachsenweger J, Jansche R, Merk T et al. ABRAXAS1 orchestrates BRCA1 activities to counter genome destabilizing repair pathways-lessons from breast cancer patients Cell death & disease 2023-05-17 [PMID: 37198153] (ICC/IF)
• Hashimoto M, Takeichi K, Murata K et al. Regulation of neural stem cell proliferation and survival by protein arginine methyltransferase 1 Frontiers in neuroscience 2022-11-10 [PMID: 36440275] (IHC-Fr, ICC/IF, Mouse)
  
  Details:
  IHC-Fr Dilution used was 1:500, ICC/IF dilution used was 1:200
• Zhu C, Iwase M, Li Z et al. Profilin-1 regulates DNA replication forks in a context-dependent fashion by interacting with SNF2H and BOD1L Nature communications 2022-11-01 [PMID: 36319634] (ICC/IF, Human)
• Chen X, Ling X, Xia J et al. Mature dendritic cell-derived dendrosomes swallow oxaliplatin-loaded nanoparticles to boost immunogenic chemotherapy and tumor antigen-specific immunotherapy Bioact Mater 2022-04-07 [PMID: 35386340]
• RodrIguez-Iglesias, N;Nadjar, A;Sierra, A;Valero, J; Susceptibility of Female Mice to the Dietary Omega-3/Omega-6 Fatty-Acid Ratio: Effects on Adult Hippocampal Neurogenesis and Glia International journal of molecular sciences [PMID: 35328825] (ICC/IF, ICC/IF, Mouse)
• Araki R, Nishida S, Oishi Y et al. Methyl Donor Supplementation Prevents a Folate Deficiency-induced Depression-like State and Neuronal Immaturity of the Dentate Gyrus in Mice Neuroscience 2022-01-19 [PMID: 35065186] (IF/IHC, Mouse)
• Tsujikawa K, Saito K, Nagasaka A, Miyata T Developmentally interdependent stretcher-compressor relationship between the embryonic brain and the surrounding scalp in the preosteogenic head Developmental dynamics : an official publication of the American Association of Anatomists 2022-01-08 [PMID: 34997665] (ICC/IF, Rat)
• Batenburg NL, Mersaoui SY, Walker JR et al. Cockayne syndrome group B protein regulates fork restart, fork progression and MRE11-dependent fork degradation in BRCA1/2-deficient cells Nucleic acids research 2021-12-16 [PMID: 34871413]
• Hattori Y, Itoh H, Tsugawa Y Et al. Embryonic pericytes promote microglial homeostasis and their effects on neural progenitors in the developing cerebral cortex The Journal of neuroscience : the official journal of the Society for Neuroscience 2021-11-24 [PMID: 34819341]
• Lim S, Kim YJ, Park S Et al. mTORC1-induced retinal progenitor cell overproliferation leads to accelerated mitotic aging and degeneration of descendent MUller glia eLife 2021-10-22 [PMID: 34677125] (IF/IHC, Mouse)
• Guo Y, Rall-Scharpf M, Bourdon JC Et al. p53 isoforms differentially impact on the POL dependent DNA damage tolerance pathway Cell death & disease 2021-10-13 [PMID: 34645785]
• He X, Smith SE, Chen S et al. Tumor-initiating stem cell shapes its microenvironment into an immunosuppressive barrier and pro-tumorigenic niche Cell reports 2021-09-07 [PMID: 34496236]
• Serpe C, Monaco L, Relucenti M Et al. Microglia-Derived Small Extracellular Vesicles Reduce Glioma Growth by Modifying Tumor Cell Metabolism and Enhancing Glutamate Clearance through miR-124 Cells 2021-08-12 [PMID: 34440835] (CellDiff, IF/IHC)
• Hambarde S, Tsai CL, Pandita RK et al. EXO5-DNA structure and BLM interactions direct DNA resection critical for ATR-dependent replication restart Molecular cell 2021-06-24 [PMID: 34197737]
• De Risi M, Tufano M, Alvino FG et al. Altered heparan sulfate metabolism during development triggers dopamine-dependent autistic-behaviours in models of lysosomal storage disorders Nature communications 2021-06-09 [PMID: 34108486] (IF/IHC, Mouse)
• Mancinelli S, Vitiello M, Donnini M et al. The Transcription Regulator Patz1 Is Essential for Neural Stem Cell Maintenance and Proliferation Frontiers in cell and developmental biology 2021-04-07 [PMID: 33898458] (Mouse)
• Karpinski BA, Maynard TM, Bryan CA et al. Selective disruption of trigeminal sensory neurogenesis and differentiation in a mouse model of 22q11.2 deletion syndrome Disease models & mechanisms 2021-05-04 [PMID: 33722956] (IF/IHC)
• Ma T, Wong SZH, Lee B et al. Decoding neuronal composition and ontogeny of individual hypothalamic nuclei Neuron 2021-02-06 [PMID: 33600763]
• Barske, L, Fabian, P Et al. Evolution of vertebrate gill covers via shifts in an ancient Pou3f3 enhancer. Proc Natl Acad Sci U S A 2020-10-06 [PMID: 32958671] (WB, IF/IHC)
• Minamide K, Sato T, Nakanishi Y et al. IRF2 maintains the stemness of colonic stem cells by limiting physiological stress from interferon Scientific reports 2020-09-08 [PMID: 32901054] (IHC-P, IF/IHC)
• Zhang Y, Li S, Li X et al. Convergent lines of evidence support NOTCH4 as a schizophrenia risk gene J. Med. Genet. 2020-09-08 [PMID: 32900838]
• Eckelmann BJ, Bacolla A, Wang H et al. XRCC1 promotes replication restart, nascent fork degradation and mutagenic DNA repair in BRCA2-deficient cells NAR Cancer 2020-09-01 [PMID: 32776008] (ICC/IF, Human)
• Gupta M, Concepcion CP, Fahey CG et al. BRG1 loss predisposes lung cancers to replicative stress and ATR dependency Cancer Res. 2020-07-20 [PMID: 32690724]
• Bai G, Kermi C, Stoy H et al. HLTF Promotes Fork Reversal, Limiting Replication Stress Resistance and Preventing Multiple Mechanisms of Unrestrained DNA Synthesis Mol. Cell 2020-05-19 [PMID: 32442397]
• Halliwell JA, Frith TJR, Price CJ et al. Nucleosides rescue replication-mediated genome instability of human pluripotent stem cells Stem Cell Reports 2020-05-16 [PMID: 32413278]
• Haglin S, Berghard A, Bohm S Increased Retinoic Acid Catabolism in Olfactory Sensory Neurons Activates Dormant Tissue-Specific Stem Cells and Accelerates Age-Related Metaplasia J. Neurosci. 2020-05-20 [PMID: 32385093] (IHC-F, Mouse)
• Nacson J, Di Marcantonio D, Wang Y et al. BRCA1 Mutational Complementation Induces Synthetic Viability Mol. Cell 2020-04-22 [PMID: 32359443]
• Silveira EJD, Nascimento Filho CHV, Yujra VQ et al. BMAL1 Modulates Epidermal Healing in a Process Involving the Antioxidative Defense Mechanism Int J Mol Sci 2020-01-30 [PMID: 32019183] (IHC-P, Mouse)
• Saito K, Okamoto M, Watanabe Y et al. Dorsal-to-Ventral Cortical Expansion Is Physically Primed by Ventral Streaming of Early Embryonic Preplate Neurons Cell Rep. 2019-11-05 [PMID: 31693895]
• Al-Dalahmah O, Campos Soares L, Nicholson J et al. Galectin-3 modulates postnatal subventricular zone gliogenesis Glia 2019-10-18 [PMID: 31626379] (IF/IHC, Human, Mouse)
• Bu F, Min JW, Munshi Y et al. Activation of endothelial ras-related C3 botulinum toxin substrate 1 (Rac1) improves post-stroke recovery and angiogenesis via activating Pak1 in mice Exp. Neurol. 2019-09-06 [PMID: 31499064]
• Kawaue T, Shitamukai A, Nagasaka A et al. Lzts1 controls both neuronal delamination and outer radial glial-like cell generation during mammalian cerebral development Nat Commun 2019-06-25 [PMID: 31239441] (IHC-Fr, Mouse)
• Voskuhl RR, Itoh N, Tassoni A et al. Waiting impulsivity during reward seeking increases adult hippocampal neurogenesis in mice Neurosci. Lett. 2019-05-19 [PMID: 31116969] (IHC-F, Mouse)
• Jia Y, Hou W, Li Y et al. The Adult Adrenal Cortex Undergoes Rapid Tissue Renewal in a Sex-Specific Manner Cell Stem Cell 2019-05-06 [PMID: 31104943] (ICC/IF, Mouse)
• Winham CL, Le T, Jellison ER et al. gamma-Synuclein Induces Human Cortical Astrocyte Proliferation and Subsequent BDNF Expression and Release Neuroscience 2019-05-10 [PMID: 31078687] (FLOW, ICC/IF, Human)
• Kyle, J;Wu, M;Gourzi, S;Tsirka, SE; Proliferation and Differentiation in the Adult Subventricular Zone Are Not Affected by CSF1R Inhibition Front Cell Neurosci 2019-04-02 [PMID: 31001085] (ICC/IF, Rat)
• Newton PT, Li L, Zhou B et al. A radical switch in clonality reveals a stem cell niche in the epiphyseal growth plate Nature 2019-03-01 [PMID: 30814736] (ICC/IF, Mouse)
• Apple DM, Mahesula S, Fonseca RS et al. Calorie restriction protects neural stem cells from age-related deficits in the subventricular zone. Aging (Albany NY) 2019-01-08 [PMID: 30622221] (IF/IHC, Mouse)
• Alves MJ, Goksel M, Kaya B et al. CCNA2 Ablation in Aged Mice Results in Abnormal rRNA Granule Accumulation in Hippocampus Am. J. Pathol. 2018-12-21 [PMID: 30579783] (ICC/IF, Mouse)
• Eckelmann B XRCC1 PLAYS DIVERSE ROLES IN DNA STRAND BREAK REPAIR AND HUMAN DISEASE Thesis 2021-01-01
• Lee CH, Kim HJ, Lee YS et al. Hypothalamic Macrophage Inducible Nitric Oxide Synthase Mediates Obesity-Associated Hypothalamic Inflammation. Cell Rep. 2018-10-23 [PMID: 30355499] (ICC/IF, Mouse)
• Nacson J, Krais JJ, Bernhardy AJ et al. BRCA1 Mutation-Specific Responses to 53BP1 Loss-Induced Homologous Recombination and PARP Inhibitor Resistance. Cell Rep 2018-09-25 [PMID: 30257212] (Human, Mouse)
• Hattori Y, Miyata T. Microglia extensively survey the developing cortex via the CXCL12/CXCR4 system to help neural progenitors to acquire differentiated properties Genes Cells 2018-08-24 [PMID: 30144249] (ICC/IF, Mouse)
• Yamada J, Nadanaka S, Kitagawa H et al. Increased synthesis of chondroitin sulfate proteoglycan promotes adult hippocampal neurogenesis in response to enriched environment J. Neurosci. 2018-08-20 [PMID: 30126967] (WB, IF/IHC, Mouse)
• Watanabe, Y, Kawaue, T Et al. Differentiating cells mechanically limit the interkinetic nuclear migration of progenitor cells to secure apical cytogenesis. Development 2018-07-17 [PMID: 29945866] (WB)
• Wang QA, Song A, Chen W et al. Reversible De-differentiation of Mature White Adipocytes into Preadipocyte-like Precursors during Lactation Cell Metab. 2018-06-08 [PMID: 29909970]
• Al Mamun A, Yu H, Romana S, Liu F. Inflammatory Responses are Sex Specific in Chronic Hypoxic-Ischemic Encephalopathy. Cell Transplant 2018-01-01 [PMID: 29692197] (Mouse)
• Padilla SL. Growing Up POMC: Pro-opiomelanocortin in the Developing Brain. Columbia University. 2011-01-01
• Lepore F, D'Alessandro G, Antonangeli F et al. CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma Front Immunol 2018-11-27 [PMID: 30542347] (ICC/IF, Mouse)
• Lin, J M, Taroc, E Z M Et al. The transcription factor Tfap2e/AP-2 epsilon plays a pivotal role in maintaining the identity of basal vomeronasal sensory neurons. Dev Biol 2018-09-01 [PMID: 29928868] (IF/IHC)
• Nam MO, Hahn S, Jee JH, Hwang TS. Effects of a small molecule R-spondin-1 substitute RS-246204 on a mouse intestinal organoid culture. Oncotarget. 2018-01-19 [PMID: 29464078] (Mouse)
• Kuroda MJ, Sugimoto C, Cai Y, Merino KM. High Turnover of Tissue Macrophages Contributes to Tuberculosis Reactivation in Simian Immunodeficiency Virus-Infected Rhesus Macaques. J. Infect. Dis. 2018-02-08 [PMID: 29432596] (Monkey)
• Kottemann MC, Conti BA, Lach FP, Smogorzewska A. Removal of RTF2 from Stalled Replisomes Promotes Maintenance of Genome Integrity. Mol. Cell 2017-12-15 [PMID: 29290612]
• Apostolopoulou M, Kiehl TR, Winter M et al. Non-monotonic Changes in Progenitor Cell Behavior and Gene Expression during Aging of the Adult V-SVZ Neural Stem Cell Niche Stem Cell Reports. 2017-11-08 [PMID: 29129683] (IHC-Fr, Mouse)
• Tang JJ, Podratz JL, Lange M et al. Mechano growth factor, a splice variant of IGF-1, promotes neurogenesis in the aging mouse brain Mol Brain 2017-07-07 [PMID: 28683812] (IHC-P, Mouse)
• Zhou B Novel skeletal stem and progenitor cells and their regulation Thesis 2022-01-01
• Sugimoto C, Merino KM, Hasegawa A, Wang X. Critical Role for Monocytes/Macrophages in Rapid Progression to AIDS in Pediatric Simian Immunodeficiency Virus-Infected Rhesus Macaques. J. Virol. 2017-09-01 [PMID: 28566378] (IF/IHC, Monkey)
• S B, K S, S A et al. RAD51 interconnects between DNA replication, DNA repair and immunity. NCBI-GEO dataSets. 2017-02-18 [PMID: 28334891] (IF/IHC, Human)
• Garofalo S, Grimaldi A, Chece G et al. The glycoside oleandrin reduces glioma growth with direct and indirect effects on tumor cells. J. Neurosci. 2017-03-14 [PMID: 28292827] (IF/IHC, Mouse)
• Huang HM, Bauer DC, Lelliott PM et al. A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice. Sci Rep. 2016-11-16 [PMID: 27848995] (IF/IHC, Mouse)
  
  Details:
  This citation used the Biotin version of this antibody.
• Parker, A, Maclaren, O J Et al. Cell proliferation within small intestinal crypts is the principal driving force for cell migration on villi. FASEB J 2017-02-01 [PMID: 27811059]
• Ishikawa R, Fukushima H, Frankland PW, Kida S. Hippocampal neurogenesis enhancers promote forgetting of remote fear memory after hippocampal reactivation by retrieval Elife 2016-09-26 [PMID: 27669409] (IF/IHC, Mouse)
• Gygli PE, Chang JC, Gokozan HN et al. Cyclin A2 promotes DNA repair in the brain during both development and aging. Aging (Albany NY). 2016-07-01 [PMID: 27425845] (WB, IF/IHC, Mouse)
• Seidel P, Remus M, Delacher M et al. Epidermal Nbn deletion causes premature hair loss and a phenotype resembling psoriasiform dermatitis. Oncotarget 2016-04-26 [PMID: 27050272] (IF/IHC)
• Guo K, Li CH, Wang XY et al. Germ stem cells are active in postnatal mouse ovary under physiological conditions. Mol. Hum. Reprod. 2016-05-01 [PMID: 26916381] (ICC/IF, Mouse)
• Feng J, Liang J, Li J et al. PTEN Controls the DNA Replication Process through MCM2 in Response to Replicative Stress. Cell Rep. 2015-11-17 [PMID: 26549452]
• Somyajit K, Saxena S, Babu S et al. Mammalian RAD51 paralogs protect nascent DNA at stalled forks and mediate replication restart. Nucleic Acids Res. 2015-09-09 [PMID: 26354865] (ICC/IF, Human)
• Lamm N, Maoz K, Bester AC et al. Folate levels modulate oncogene-induced replication stress and tumorigenicity EMBO Mol Med. 2015-07-21 [PMID: 26197802] (Human)
• Zhu J, Su F, Mukherjee S et al. FANCD2 influences replication fork processes and genome stability in response to clustered DSBs. Cell Cycle 2015-01-01 [PMID: 26083937] (WB)
• Lee Rj, Kim Jk, Chao D et al. Progesterone and allopregnanolone improve stroke outcome in male mice via distinct mechanisms but neither promotes neurogenesis J. neurochem. 2014-11-07 [PMID: 25376903] (IHC-Fr)
• Tong CK, Han YG, Shah JK et al. Primary cilia are required in a unique subpopulation of neural progenitors. Proc. Natl. Acad. Sci. U.S.A. 2014-08-26 [PMID: 25114218] (IHC-P, Mouse)
• Quadrato G, Elnaggar MY, Duman C et al. Modulation of GABAA Receptor Signaling Increases Neurogenesis and Suppresses Anxiety through NFATc4. J. Neurosci. 2014-06-18 [PMID: 24948817] (IHC-FrFl)
  
  Details:
  Tissue samples of Wild-type (WT) and nuclear factor of activated T cell-knock-out (NFATc4?/?) mice that were maintained on a mixed C57BL/6 and 129S7 genetic background and injected with BrdU 75 mg/kg body weight in saline or other treatiments (muscimol/temozolomide) were used for IHC-FrFl as - mice perfused transcardially with 0.1 M PBS solution, pH 7.4, followed by 4% PFA-PBS pH 7.4, brains dissected /postfixed O/N 4C, immersed in 30% sucrose and coronal free-floating cryosections of 40 um spanning the whole hippocampus cut and stained using rat monoclonal antibody anti-BrdU (1:100; Novus Biologicals).
• Diensthuber Marc, Zecha Veronika, Wagenblast Jens et al. Spiral ganglion stem cells can be propagated and differentiated into neurons and glia. Biores Open Access. 2014-06-01 [PMID: 24940560]
• Kawaue T, Sagou K, Kiyonari H et al. Neurogenin2-d4Venus and Gadd45g-d4Venus transgenic mice: Visualizing mitotic and migratory behaviors of cells committed to the neuronal lineage in the developing mammalian brain. Dev. Growth Differ. 2014-05-01 [PMID: 24712911] (IHC-Fr)
• Ishikawa R, Kim R, Namba T et al. Time-dependent enhancement of hippocampus-dependent memory after treatment with memantine: Implications for enhanced hippocampal adult neurogenesis. Hippocampus 2014-03-06 [PMID: 24599753] (IHC-Fr)
• Nobs L, Baranek C, Nestel S et al. Stage-specific requirement for cyclin D1 in glial progenitor cells of the cerebral cortex. Glia 2014-02-19 [PMID: 24550001] (IHC-Fr)
• Cai Y, Sugimoto C, Arainga M et al. In vivo characterization of alveolar and interstitial lung macrophages in rhesus macaques: Implications for understanding lung disease in humans. J Immunol 2014-03-15 [PMID: 24534529] (IHC-P)
• Conway Anthony, Schaffer David V. Biomaterial microenvironments to support the generation of new neurons in the adult brain. Stem Cells. 2014-05-01 [PMID: 24449485]
• Toko H, Hariharan N, Konstandin MH et al. Differential Regulation of Cellular Senescence and Differentiation by Prolyl Isomerase Pin1 in Cardiac Progenitor Cells. J Biol Chem 2013-12-27 [PMID: 24375406] (FLOW)
• Di Giovannantonio LG, Di Salvio M, Omodei D et al. Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity. Development 2014-01-01 [PMID: 24335253] (IHC-P, Mouse)
• Xie M, Yen Y, Owonikoko TK et al. Bcl2 induces DNA replication stress by inhibiting ribonucleotide reductase. Cancer Res. 2014-01-01 [PMID: 24197132] (ICC/IF)
• Harmacek L, Watkins-Chow DE, Chen J et al. A unique missense allele of BAF155, a core BAF chromatin remodeling complex protein, causes neural tube closure defects in mice. Dev Neurobiol. 2013-10-29 [PMID: 24170322] (ICC/IF, IF/IHC, Mouse)
• Elabd C, Cousin W, Chen RY et al. DNA methyltransferase-3-dependent nonrandom template segregation in differentiating embryonic stem cells. J Cell Biol. 2013-10-14 [PMID: 24127215] (ICC/IF, Human)
• Yamada J, Jinno S. et al. S100A6 (calcyclin) is a novel marker of neural stem cells and astrocyte precursors in the subgranular zone of the adult mouse hippocampus. Hippocampus 2014-01-01 [PMID: 24115312] (IHC-Fr)
• Smardencas A, Rizkalla K, Kim HA et al. Phenotyping dividing cells in mouse models of neurodegenerative basal ganglia diseases. BMC Neurosci. 2013-10-03 [PMID: 24090101] (IHC-Fr, ICC/IF, Mouse)
• Okamoto M, Namba T, Shinoda T et al. TAG-1-assisted progenitor elongation streamlines nuclear migration to optimize subapical crowding. Nat Neurosci. 2013-11-01 [PMID: 24056697] (ICC/IF, IHC-Fr)
• Kosaka K, Kosaka T. Secretagogin-containing neurons in the mouse main olfactory bulb. Neurosci Res. 2013-09-02 [PMID: 24008127] (IF/IHC, ICC/IF)
• Forsberg K, Wuttke A, Quadrato G et al. The Tumor Suppressor p53 Fine-Tunes Reactive Oxygen Species Levels and Neurogenesis via PI3 Kinase Signaling. J Neurosci. 2013-09-04 [PMID: 24005285] (ICC/IF, IHC-Fr)
• Das I, Park JM, Shin JH et al. Hedgehog agonist therapy corrects structural and cognitive deficits in a Down syndrome mouse model. Sci Transl Med. 2013-09-04 [PMID: 24005160]
• Chang GQ, Karatayev O, Leibowitz SF. Prenatal exposure to nicotine stimulates neurogenesis of orexigenic Peptide-expressing neurons in hypothalamus and amygdala. J Neurosci. 2013-08-21 [PMID: 23966683] (IF/IHC, ICC/IF, Rat)
• Nobs L, Nestel S, Kulik A et al. Cyclin D1 is required for proliferation of olig2-expressing progenitor cells in the injured cerebral cortex. Glia 2013-09-01 [PMID: 23839966] (IF/IHC, ICC/IF)
• Cuccurazzu B, Bortolotto V, Valente MM et al. Upregulation of mGlu2 Receptors via NF-kB p65 Acetylation Is Involved in the Proneurogenic and Antidepressant Effects of Acetyl-L-Carnitine. Neuropsychopharmacology 2013-05-14 [PMID: 23670591] (ICC/IF, IF/IHC, Mouse)
• King SL, Mohiuddin JJ, Dekaney CM et al. Paneth cells expand from newly created and preexisting cells during repair after doxorubicin-induced damage. Am J Physiol Gastrointest Liver Physiol 2013-07-01 [PMID: 23660502] (IHC-P)
• El-Gohary Y, Tulachan S, Guo P et al. Smad signaling pathways regulate pancreatic endocrine development. Dev Biol 2013-04-17 [PMID: 23603491] (IHC-Fr, ICC/IF)
• Meneghini V, Bortolotto V, Francese MT et al. High-Mobility Group Box-1 Protein and beta-Amyloid Oligomers Promote Neuronal Differentiation of Adult Hippocampal Neural Progenitors via Receptor for Advanced Glycation End Products/Nuclear Factor-B Axis: Relevance for Alzheimer's Disease J Neurosci 2013-04-03 [PMID: 23554486] (ICC/IF, IF/IHC, Mouse)
• Weiss RM, Guo S, Shan A et al. Brg1 Determines Urothelial Cell Fate during Ureter Development. J Am Soc Nephrol 2013-02-28 [PMID: 23449535]
• Ponti G, Obernier K, Guinto C et al. Cell cycle and lineage progression of neural progenitors in the ventricular-subventricular zones of adult mice. Proc Natl Acad Sci U S A 2013-03-12 [PMID: 23431204] (IF/IHC, Mouse)
• Powers BE, Sellers DL, Lovelett EA et al. Remyelination reporter reveals prolonged refinement of spontaneously regenerated myelin. Proc Natl Acad Sci U S A 2013-03-05 [PMID: 23431182] (IHC-Fr, ICC/IF, Mouse)
• Choe Y, Pleasure SJ. Wnt Signaling Regulates Intermediate Precursor Production in the Postnatal Dentate Gyrus by Regulating Cxcr4 Expression. Dev Neurosci 2012-12-14 [PMID: 23257686] (IHC-Fr, ICC/IF, Mouse)
• Wu X, Walters TJ, Rathbone CR. Skeletal muscle satellite cell activation following cutaneous burn in rats Burns 2012-11-09 [PMID: 23146573] (ICC/IF)
• Neal MD, Sodhi CP, Jia H et al. Toll-like Receptor 4 Is Expressed on Intestinal Stem Cells and Regulates Their Proliferation and Apoptosis via the p53 Up-regulated Modulator of Apoptosis J Biol Chem 2012-10-26 [PMID: 22955282] (ICC/IF, IF/IHC)
• Chang GQ, Karatayev O, Liang SC et al. Prenatal ethanol exposure stimulates neurogenesis in hypothalamic and limbic peptide systems: Possible mechanism for offspring ethanol overconsumption Neuroscience 2012-06-25 [PMID: 22742906] (IF/IHC, ICC/IF, Rat)
• Tien AC, Tsai HH, Molofsky AV et al. Regulated temporal-spatial astrocyte precursor cell proliferation involves BRAF signalling in mammalian spinal cord Development 2012-07-01 [PMID: 22675209] (IF/IHC, ICC/IF, Mouse)
• Quadrato G, Benevento M, Alber S, Jacob C, Floriddia EM, Nguyen T, Elnaggar MY, Pedroarena CM, Molkentin JD, Di Giovanni S. Nuclear factor of activated T cells (NFATc4) is required for BDNF-dependent survival of adult-born neurons and spatial memory formation in the hippocampus. Proc Natl Acad Sci U S A. 2012-05-14 [PMID: 22586092] (IF/IHC, Mouse)
• Bye CR, Thompson LH, Parish CL. Birth dating of midbrain dopamine neurons identifies A9 enriched tissue for transplantation into Parkinsonian mice. Exp Neurol. 2012-04-16 [PMID: 22524988] (IF/IHC, ICC/IF, Mouse)
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Reviews for Bromodeoxyuridine/BrdU Antibody (NB500-169) (9)

Average Rating: 4.8

(Based on 9 reviews)

We have 9 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 04/18/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Olfactory epithelium sections
• Species: Mouse
• Lot: 1015

Comments

• Comments: Works great with heat antigen retrieval or HCl. No signal without retrieval.

reviewed by: Imanol Gonzalez Burguera ICC Human 10/27/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: NT2 cell line,NT2 cells
• Species: Human
• Lot: 1706-6 + 1706-7

Comments

• Comments: Human teratocarcinoma NTERA2-D1 (NT2); cells from American Type Culture Collection (ATCC®, CRL-1973™); were seeded in poly D-Lysine treated and Matrigel™ coated glass coverslips and maintained in complete medium (DMEM®, supplemented with 10% FBS and antibiotics).
  
  BrdU antigen retrieval protocol:
  1.     Fix cells with PF 4% as usual.
  2.     Permeabilize cells during 20 min RT with PBS-T.
  3.     2 h at 65 ºC in 50% Formamide (protected from light).
  4.     Wash twice (5’ each) with SSC 1X.
  5.     30’ at 37 ºC in 2N HCl.
  6.     10’ at RT in 0.1 M Sodium borate, pH 8.5 for neutralization.
  7.     Wash twice 5' each with PBS-T.
  
  Primary antibody concentration: 1:250
  
  Secondary Antibody: GARat Alexa 568 (ref: A11077 invitrogen) 1:400

reviewed by: Verified Customer ICC Human 06/07/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: DAN fiber spreads of head and neck cancer cells
• Species: Human
• Lot: 1015

Comments

• Comments: Comments: We routinely use product # NB500-169 in performing DNA fiber assay. This product works great for visualizing incorporated CldU into the DNA of proliferating cancer cells. This product has a high signal to noise ratio. Overall, we rate this product highly for immunofluorescent detection of ClDU in DNA fiber assay.
  Blocking details: 10% Normal goat serum + 0.1% Triton X-100 in PBS for 1hr at room temperature
  Primary Antibody (# NB500-169): 1:200 in blocking buffer for 1:30 hrs in humidified chamber
  Secondary Antibody: Alexa fluor 555 goat anti-rat, 1:300 in blocking buffer for 1hr in humidified chamber
  Detection: Fluorescent microscopy
  Fixation and Denaturation details: Cells are lysed in lysis buffer (1% SDSA, 50 mM EDTA, 200 mM Tris-Cl), and spotted on glass slide. Glass slide is tilted to enable DAN spreading. Upon air drying, DNA is fixed in methanol:acetonbe ( 3:1) for 15 min and then denatured in 2.5 N HCl, then neutralized in PBS (pH 8).

reviewed by: Adrienne Franks IHC Mouse 04/27/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: Mouse Pancreas
• Species: Mouse

reviewed by: Adrienne Franks IHC Mouse 04/18/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: mouse liver,Mouse Pancreas,Mouse Spleen
• Species: Mouse

reviewed by: Verified Customer ICC Mouse 04/02/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Adult mouse brain,adult mouse neural stem cells,Mouse brain,Mouse embryo
• Species: Mouse
• Lot: N/A

reviewed by: Verified Customer IHC-P Mouse 05/29/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: BrdU-treated mouse duodenum
• Species: Mouse

reviewed by: Valeria Bortolotto IHC-Fr Mouse 08/07/2013

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: mouse brain
• Species: Mouse
• Lot: 1210109

reviewed by: Donald Maris IHC-P Mouse 04/14/2010

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: mouse brain
• Species: Mouse
• Lot: IMAS252072

FAQs for Bromodeoxyuridine/BrdU Antibody (NB500-169). (Showing 1 - 3 of 3 FAQs).

1. I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5
   • We recommend this product: NB500-235​
2. Is the NB500-169 still available in the concentration of 2mg/ml? We ordered five vials in the past month with the unit size 0.1 mg. Three of them arrived with a concentration of 2mg/ml, the last two vial with a concentration of 1mg/ml. Can we influence the concentration when ordering?
   • In terms of the concentration of the vials, unfortunately sometimes the concentrations of our products may change. (The concentration of each vial is that stated on the label.) I am sorry, but it is not possible for you to order specifically 2 mg/ml vials of NB500-169. (If you require a concentration of 2 mg/ml, you may be interested in our Antibody Concentration and Clean Up Kit (product code for 1 spin column is 861-0005; 3 spin columns is 861-0010), which you could use to reduce the volume of the antibody by half and hence double the concentration.)
3. I am planning on doing DNA fiber fish using: 5'-iodo-2-deoxyuridine (IdU) and 5'-chloro-2-deoxiuridine (CldU). What antibodies from Novus do you recommend?
   • Catalog number NB500-169 is your best candidate for IdU as it has been very well supported by 52 references. As far as antibodies for CIdU is concerned, unfortunately currently we do not carry any antibodies for it however because of the high similarity of CldU and IdU with BrdU, the anti-BrdU antibodies also react with these modified nucleosides and you can totally use NB500-169 to detect both IdU and CldU, the only problem is, this might complicate the multiple labeling of cells.