BSA Removal Antibody Purification Kit

Western Blot: BSA Removal Antibody Purification Kit [820-0100]

• Applications: WB

BSA Removal Antibody Purification Kit Summary

• Description: Bovine Serum Albumin (BSA) is often added to purified antibodies as it is an effective stabilizer. However, when labeling antibodies, the BSA becomes a hindrance, as it directly competes with the antibody to attach to the label, greatly reducing the conjugation efficiency. Therefore, prior to undertaking labeling techniques, it is essential to remove the BSA.
  
  Common commercial BSA removal techniques can involve many laborious steps. The AbSelect BSA Removal Kit is a simple 1 step, 10 minute method which effectively separates the BSA from the antibody and can be used on any antibody sub-type or species.
  
  Benefits:
  • Easy-to-use
  • Only takes 10 minutes!
  • Centrifuge is the only equipment required
  • Also use to concentrate and exchange your buffer
• Kit Type: Antibody Purification Kit

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: The AbSelectTM BSA Removal Kit can separate BSA from antibody solutions with antibody concentrations from 0.03mg/ml to 10mg/ml. Separation is more efficient at higher antibody concentrations. BSA can be effectively separated when present at concentrations of up to 0.5%. If BSA is present at higher concentrations, dilute the antibody mix with de-ionised, distilled water until BSA concentration is 0.5% or less.

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Background

Bovine Serum Albumin (BSA) is often added to purified antibodies as it is an effective stabilizer. However, when labeling antibodies, the BSA becomes a hindrance, as it directly competes with the antibody to attach to the label, greatly reducing the conjugation efficiency. Therefore, prior to undertaking labeling techniques, it is essential to remove the BSA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.