CA125/MUC16 Antibody (CL2782) - BSA Free

Immunohistochemistry-Paraffin: CA125/MUC16 Antibody (CL2782) [NBP2-59023] - Analysis in human cervix, uterine and liver tissues using NBP2-59023 antibody. Corresponding MUC16 RNA-seq data are presented for the same tissues.

Immunocytochemistry/Immunofluorescence: CA125/MUC16 Antibody (CL2782) [NBP2-59023] - Staining of HeLa cells using the Anti-MUC16 monoclonal antibody, showing specific staining in the plasma membrane and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: CA125/MUC16 Antibody (CL2782) [NBP2-59023] -Staining of human cervix, uterine shows moderate membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: CA125/MUC16 Antibody (CL2782) [NBP2-59023] -Staining of human fallopian tube shows moderate positivity in glandular cells.

Immunohistochemistry-Paraffin: CA125/MUC16 Antibody (CL2782) [NBP2-59023] -Staining of human endometrium shows strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: CA125/MUC16 Antibody (CL2782) [NBP2-59023] -Staining of human liver shows no positivity in hepatocytes as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clone: CL2782
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

CA125/MUC16 Antibody (CL2782) - BSA Free Summary

• Immunogen: This antibody was developed using a recombinant protein derived from Q8WXI7, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUC16
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for CA125/MUC16 Antibody (CL2782) - BSA Free

• CA125 ovarian cancer antigen
• CA125
• CA-125
• CA125MUC-16
• FLJ14303
• MUC16
• mucin 16, cell surface associated
• mucin-16
• Ovarian cancer-related tumor marker CA125
• Ovarian carcinoma antigen CA125

Background

MUC16 (CA125) is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is over expressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller "subunits" being described by some investigators.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CA125/MUC16 Antibody (NBP2-59023)(1)

Publication using NBP2-59023

• Victoria O. Shender, Ksenia S. Anufrieva, Polina V. Shnaider, Georgij P. Arapidi, Marat S. Pavlyukov, Olga M. Ivanova, Irina K. Malyants, Grigory A. Stepanov, Evgenii Zhuravlev, Rustam H. Ziganshin, Ivan O. Butenko, Olga N. Bukato, Ksenia M. Klimina, Vladimir A. Veselovsky, Tatiana V. Grigorieva, Sergey Y. Malanin, Olga I. Aleshikova, Andrey V. Slonov, Nataliya A. Babaeva, Lev A. Ashrafyan, Elena Khomyakova, Evgeniy G. Evtushenko, Maria M. Lukina, Zixiang Wang, Artemiy S. Silantiev, Anna A. Nushtaeva, Daria D. Kharlampieva, Vassili N. Lazarev, Arseniy I. Lashkin, Lorine K. Arzumanyan, Irina Yu. Petrushanko, Alexander A. Makarov, Olga S. Lebedeva, Alexandra N. Bogomazova, Maria A. Lagarkova, Vadim M. Govorun Therapy-induced secretion of spliceosomal components mediates pro-survival crosstalk between ovarian cancer cells Nature Communications 2024-06-19 [PMID: 38898005]

Bioinformatics

• Gene Symbol: MUC16
• Uniprot:
  • Q8WXI7()