CA125/MUC16 Antibody (X306cc) - BSA Free

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA, MA
• Clone: X306cc
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

CA125/MUC16 Antibody (X306cc) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with CA125 antigen purified from human ovarian carcinoma (MW > 1 MDa).
• Epitope: A1 epitope group
• Localization: Cell membrane; Single pass type I membrane protein. Secreted, extracellular space.
• Specificity: CA125 antigen. Epitope specificity group A (ISOBM classification) similar to OC125.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUC16
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Microarray
  • Sandwich ELISA 1:100-1:2000
  • Simple Western 1:50
  • Western Blot 1:100-1:2000
• Application Notes: Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection): NB120-10032 (capture) with NBP2-11624 or NB600-1468 as detection. Microarray was reported in scientific literature. Use in ELISA reported in scientific literature (PMID 24257318)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for CA125/MUC16 Antibody (X306cc) - BSA Free

• CA125 ovarian cancer antigen
• CA125
• CA-125
• CA125MUC-16
• FLJ14303
• MUC16
• mucin 16, cell surface associated
• mucin-16
• Ovarian cancer-related tumor marker CA125
• Ovarian carcinoma antigen CA125

Background

CA125/MUC16 antigen is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that CA125/MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CA125/MUC16 Antibody (NB120-10032)(5)

Publications using NB120-10032

• Choi, M;Tichenor, AA; Regional Conjunctival Differences in Glycocalyx Mucin Expression in Dry Eye and Normal Subjects Investigative ophthalmology & visual science 2024-02-01 [PMID: 38334701]
• Tang H, Partyka K, Hsueh P et al. Glycans Related to the CA19-9 Antigen Are Increased in Distinct Subsets of Pancreatic Cancers and Improve Diagnostic Accuracy Over CA19-9. CMGH. 2015-12-20 [PMID: 26998508] (Microarray, Human)
• Gold DV, Newsome G, Liu D et al. Mapping PAM4 (clivatuzumab), a monoclonal antibody in clinical trials for early detection and therapy of pancreatic ductal adenocarcinoma, to MUC5AC mucin. Mol Cancer. 2013-11-20 [PMID: 24257318] (ELISA, Human)
• The Marker State Space (MSS) method for classifying clinical samples. Fallon BP, Curnutte B, Maupin KA et al. PLoS One [PMID: 23750276] (MA, Human)
• Cao Z, Partyka K, McDonald M et al. Modulation of glycan detection on specific glycoproteins by lectin multimerization. Anal Chem 2013-02-05 [PMID: 23286506] (MiAr, Human)

FAQs for CA125/MUC16 Antibody (NB120-10032). (Showing 1 - 2 of 2 FAQs).

1. We are setting up an ELISA type of assay for CA125. On your web page: Applications: Uses: Detection of CA125 antigen. Suggested pairs for sandwich immunoassay (capture-detection): NB120-10032 (capture) with NBP2-11624 or NB600-1468 as detection. Can you send me more details how these antibodies have been working in your sandwich immunoassay?
   • These antibodies have been tested with very general sandwich ELISA, without any assay optimization, just to determine which MAbs work together as pairs. Coat plate with NB120-10032 in alkaline carbonate buffer Post-coat with casein Add HRP-conjugated NBP2-11624 and antigen to plate Incubate microplates for 45 min at room temperature with shaking Wash 5 times with saline-Tween Add 100 ul of substrate Stop enzymatic reaction addition of 5% sulphuric acid. Measure the optical density. The sensitivity without optimization has been around 5 IU/ml. But we really do not have any recommended protocol and the suitability and sensitivity of the Abs should be determined in your own assay.
2. I need to order 3 batches of this antibody with a concentration near to 9 mg/mL, and I would like to know if it is possible to receive the product with this concentration?
   • We unfortunately do not have any lots ready that are available at 9.0mg/ml. This is what we have currently: Lot: 12/07-C29-X306, conc. 4.1 mg/ml, Lot: 12/08-C29-X306, conc. 6.4 mg/ml, Lot: 13/01-C29-X306, conc. 3.1 mg/ml. We have our productions purified off site and receive the units in at their given concentrations. Unless you are interested in purchasing a bulk amount, we will only be able offer you the product we have currently available.

Bioinformatics

• Gene Symbol: MUC16
• Entrez:
  • 94025(Human)
• Uniprot:
  • Q8WXI7()