Calcein AM Cell Viability Assay Kit (Fluorometric)

Product Discontinued

Calcein AM Cell Viability Assay Kit (Fluorometric) Summary

• Description: Convenient, non-radioactive and high-throughput assay for quantitating cell viability, cell proliferation, chemotaxis, cytotoxicity and apoptosis. Detects as few as 50 viable cells in less than 30 min.
  
  Detection method: fluorescence (Ex/Em = 485/530 nm) Applications: Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Assessment of physiological mediators and antibodies that affect cell growth.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab228556)
• Kit Type: Assay Kit (Fluorometric)

Packaging, Storage & Formulations

• Storage: Store at -20C.

Background

Quantification of number of viable cells is an indispensible tool in Cell Biology research. The Calcein AM Cell Viability Assay Kit is a fluorometric method for extremely sensitive quantification of viable cells that can detect as few as 50 viable cells in less than 30 min. Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. Hydrolysis of Calcein AM by intracellular esterase produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.