Calmodulin Antibody (6D4)

Western Blot: Calmodulin Antibody (6D4) [NB120-5494] - Analysis of purified calmodulin using NB120-2860 (right) and NB120-5494 (left).

Product Discontinued

Calmodulin Antibody (6D4) Summary

• Immunogen: Calmodulin purified from Dictyostelium discoideum.
• Localization: Cytoplasmic and Plasma membrane
• Specificity: This does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALM1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Western Blot 1:2000
• Application Notes: WB: Detects an approx. 17 kDA protein representing Calmodulin from Dictyostelium cell lysate. Addition of EGTA to buffers completely inhibits antibody staining. IHC: Staining of calmodulin in Dictyostelium cells with ab5494 results in staining of the contractile vacuoles.

Reactivity Notes

Chlamydomonas and Dictyostelium.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Calmodulin Antibody (6D4)

• CALM
• CALM1
• CALM2
• CALM3
• CALML2
• calmodulin 1 (phosphorylase kinase, delta)
• Calmodulin 1
• calmodulin
• CaM
• CAM1
• CAM2
• CAM3
• CAMB
• CAMC
• CAMI
• CAMIII
• CPVT4
• DD132
• PHKDCAM
• phosphorylase kinase, delta subunit

Background

Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Calmodulin Antibody (NB120-5494). (Showing 1 - 1 of 1 FAQ).

1. I am looking for an antibody that can bind an engineered calmodulin domain of a fluorescent biosensor. Can you provide me the amino acid sequence that your calmodulin antibodies were raised against so that I can compare them to the sequence I am working with? Can you please provide your sequence identity for this fragment: D Q L T E E Q I A E F K E A F S L F D K D G D G T I T T K E L G T V M R S L G Q N P T E A E L Q D M I N E V D A D G D G T I D F P E F L T M M A R K M W G T D S E E E I R E A F R V F D K D G N G Y I G A A E L R H V M A N L G E R L T D E E V D E M I R V A D I N G D G Q V S Y E E F V Q M M T A K
   • Please see this link to our available calmodulin antibodies. This sequences shares 94% similarity with human calmodulin and does not share 100% similarity with any known calmodulin sequence. Any of our calmodulin antibodies will be predicted to react with this sequence. Any of our polyclonal antibodies will be a good choice and chances are most of the monoclonal antibodies will cross-react as well although, unfortunately, there is no way to predict this with the monoclonals. NBP1-61548 would be my best recommendation for your assay and sequence.

Bioinformatics

• Gene Symbol: CALM1
• Entrez:
  • 24242(Rat)