Calpain 3 Antibody

Immunohistochemistry-Paraffin: Calpain 3 Antibody [NBP2-34061] - Staining of human skeletal muscle shows high expression.

Immunohistochemistry-Paraffin: Calpain 3 Antibody [NBP2-34061] - Staining of human pancreas shows low expression as expected.

Immunohistochemistry-Paraffin: Calpain 3 Antibody [NBP2-34061] - Staining in human skeletal muscle and pancreas tissues using anti-CAPN3 antibody. Corresponding CAPN3 RNA-seq data are presented for the same tissues.

Product Discontinued

Calpain 3 Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: SEEVENTISVDRPVKKKKTKPIIFVSDRANSNKELGVDQESEEGKGKTSPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQIAGDDMEICADELKKVL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAPN3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Calpain 3 Antibody

• calpain 3, (p94)
• Calpain L3
• Calpain p94
• calpain, large polypeptide L3
• calpain-3
• CANP 3
• CANP3MGC4403
• CANPL3
• EC 3.4.22
• EC 3.4.22.54
• LGMD2
• LGMD2A
• MGC10767
• MGC11121
• MGC14344
• muscle-specific calcium-activated neutral protease 3 large subunit
• Muscle-specific calcium-activated neutral protease 3
• NCL1
• nCL-1large [catalytic] subunit
• New calpain 1

Background

Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAPN3
• Uniprot:
  • P20807(Human)