CaM Kinase II delta Antibody (1A8) - Azide and BSA Free

Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of CAMK2D expression in transfected 293T cell line by CAMK2D monoclonal antibody (M02), clone 1A8.Lane 1: CAMK2D transfected lysate(54.1 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of monoclonal antibody to CAMK2D on NIH/3T3 cell. Antibody concentration 10 ug/ml.

Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - CAMK2D monoclonal antibody (M02), clone 1A8 Western Blot analysis of CAMK2D expression in NIH/3T3 ( Cat # L018V1 ).

Western Blot: CaM Kinase II delta Antibody (1A8) [H00000817-M02] - Analysis of CAMK2D over-expressed 293 cell line, cotransfected with CAMK2D Validated Chimera RNAi ( Cat # H00000817-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK2D monoclonal antibody (M02) clone 1A8 (Cat # H00000817-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, KD
• Clone: 1A8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CaM Kinase II delta Antibody (1A8) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CAMK2D (AAH32784, 301 a.a. ~ 410 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTAFEPEALGNLVEGMDFHRFYFENALSKSNKPI
• Specificity: CAMK2D (1A8)
• Isotype: IgG2b Lambda
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAMK2D
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Knockdown Validated
  • Western Blot 1:100-1:2000
• Application Notes: Antibody reactivity against recombinant protein and cell lysate for WB. It has also been used for IF, RNAi Validation and ELISA.
• Control:

  NIH 3T3 Whole Cell Lysate

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Rat reported in scientific literature (PMID: 22766271).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CaM Kinase II delta Antibody (1A8) - Azide and BSA Free

• calcium/calmodulin-dependent protein kinase (CaM kinase) II delta
• calcium/calmodulin-dependent protein kinase II delta
• calcium/calmodulin-dependent protein kinase type II delta chain
• calcium/calmodulin-dependent protein kinase type II subunit delta
• CaM kinase II delta subunit
• CaM Kinase II delta
• CaM kinase II subunit delta
• CAMK2D
• CAMKD
• CaMK-II delta subunit
• CaMK-II subunit delta
• CaM-kinase II delta chain
• DKFZp686G23119
• DKFZp686I2288
• EC 2.7.11
• EC 2.7.11.17
• MGC44911

Background

The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Four alternatively spliced transcript variants that encode three different isoforms have been characterized to date. Distinct isoforms of this chain have different expression patterns.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CaM Kinase II delta Antibody (H00000817-M02)(3)

Publications using H00000817-M02

• Hula N, Spaans F, Jennie V, et al. Placental Treatment Improves Cardiac Tolerance to Ischemia/Reperfusion Insult in Adult Male and Female Offspring Exposed to Prenatal Hypoxia Pharmacological research 2021-01-26 [PMID: 33513355] (WB, Rat)
• Arantes LA, Aguiara CJ, Amaya MJ et al. Nuclear Inositol 1,4,5-Trisphosphate Is A Necessary And Conserved Signal For The Induction Of Both Pathological And Physiological Cardiomyocyte Hypertrophy J Mol Cell Cardiol 2012-07-02 [PMID: 22766271] (WB, Rat)
• Hula N Developmental Programming of Cardiac and Coronary Artery Dysfunction in Adult Male and Female Offspring Exposed to Prenatal Hypoxia Thesis 2023-01-01 [PMID: 22348036] (WB, Rat)

Reviews for CaM Kinase II delta Antibody (H00000817-M02) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Rat.

reviewed by: Verified Customer WB Rat 08/07/2019

Summary

• Application: Western Blot
• Sample Tested: Heart lysates
• Species: Rat
• Lot: H7201-1A8

Comments

• Comments: Adult rat heart lysates, reduced and denatured, resolved on 12% SDS-acrylamide gel. Transfer onto 0.22 um nitrocellulose membrane. Block with Rockland blocking solution. incubate membrane overnight at 4C with 1:100 dilution of CaMKII-delta antibody. wash with PBS-0.1% tween 20. incubate for one hour at room temp with 1:10 000 LiCor IRDye 800CW donkey anti-mouse IgG (H+L). Images taken with Odyssey V3.0.

reviewed by: Anne Frei WB Human 02/11/2015

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysates
• Species: Human

Bioinformatics

• Gene Symbol: CAMK2D
• Entrez:
  • 817(Human)
  • 108058(Mouse)
  • 24246(Rat)
• OMIM:
  • 607708(Human)
• Uniprot:
  • AAH32784(Human)