Canine Parvovirus Antibody (2G10A3) - BSA Free

Western Blot: Canine Parvovirus Antibody (2G10A3) [NBP2-61721] - Analysis using CPV-VP2 mAb against HEK293 (1) and CPV-VP2 (AA: 1-128)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: Canine Parvovirus Antibody (2G10A3) [NBP2-61721] - Analysis of Hela cells using CPV-VP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry-Paraffin: Canine Parvovirus Antibody (2G10A3) [NBP2-61721] - Parvovirus immunoreactivity in canine heart. Primary antibody used at 1ug/mL and incubated for 30 minutes at room temperature. IHC-P image submitted by a verified customer review.

Flow Cytometry: Canine Parvovirus Antibody (2G10A3) [NBP2-61721] - Analysis of Hela cells using CPV-VP2 mouse mAb (green) and negative control (red).

Western Blot: Canine Parvovirus Antibody (2G10A3) [NBP2-61721] - Analysis using CPV-VP2 mAb against Canine CPV-VP2 (AA: 1-128) recombinant protein. (Expected MW is 39.4 kDa)

Western Blot: Canine Parvovirus Antibody (2G10A3) [NBP2-61721] - Analysis using CPV-VP2 mouse mAb against SW480 (1), A431 (2), K562 (3), and BCBL-1 (4) cell lysate.

Immunohistochemistry-Paraffin: Canine Parvovirus Antibody (2G10A3) [NBP2-61721] - Analysis of cervical cancer tissues using CPV-VP2 mouse mAb with DAB staining.

Immunohistochemistry-Paraffin: Canine Parvovirus Antibody (2G10A3) [NBP2-61721] - Analysis of colon cancer tissues using CPV-VP2 mouse mAb with DAB staining.

ELISA: Canine Parvovirus Antibody (2G10A3) [NBP2-61721] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu, V-Vi
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 2G10A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Canine Parvovirus Antibody (2G10A3) - BSA Free Summary

• Immunogen: Purified recombinant fragment of Canine Parvovirus Canine Parvovirus (AA: 1-128) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 -1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 64.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Canine Parvovirus Antibody (2G10A3) - BSA Free

• CPV
• Parvo

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Canine Parvovirus Antibody (NBP2-61721)(1)

Publication using NBP2-61721

• Miao B, Chen S, Zhang X et al. T598 and T601 phosphorylation sites of canine parvovirus NS1 are crucial for viral replication and pathogenicity Veterinary microbiology 2022-01-01 [PMID: 34915313] (ICC/IF, Virus)

Review for Canine Parvovirus Antibody (NBP2-61721) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Canine.

reviewed by: Verified Customer IHC-P Canine 11/01/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Heart tissue
• Species: Canine
• Lot: 160630