Human Carbohydrate Antigen 50 ELISA Kit (Colorimetric)

Human Carbohydrate Antigen 50 ELISA Kit (Colorimetric) [NBP2-59964] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell culture supernates, ascitic fluid, tissue homogenates and cell lysates
• Standard Curve Range: 10 U/mL - 120 U/mL
• Sensitivity: 2.5 U/mL

Human Carbohydrate Antigen 50 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CA50. No significant cross-reactivity or interference between human CA50 and analogues was observed.
• Standard Curve Range: 10 U/mL - 120 U/mL
• Sensitivity: 2.5 U/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 20
• Intra-Assay: %CV < 15
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Carbohydrate Antigen 50 ELISA Kit (Colorimetric)

• CA50

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antibody specific for CA50. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for CA50 and incubated. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA50 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.