Carboxymethyl Lysine Antibody (318003)

Western blot shows 100-fold diluted samples of sera from control, Type 1 diabetic, and Type 2 diabetic volunteers. PVDF membrane was probed with 1 µg/mL Carboxymethyl Lysine Monoclonal Antibody (Catalog # MAB3247) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Greater band intensity for the IgG heavy chain (IgG hc) suggests increased glycation in diabetic sera. For additional reference, carboxymethyl lysine-adducted (CML) Leptin and carboxyethyl lysine-adducted (CEL) Leptin were included. A specific band for CML-adducted Leptin was detected (as indicated). This experiment was conducted under reducing conditions using Immunoblot Buffer Group 1.

• Reactivity: All-Multi
• Applications: WB
• Clone: 318003
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Carboxymethyl Lysine Antibody (318003) Summary

• Immunogen: Carboxymethyl Lysine-modified Keyhole Limpet Hemocyanin (KLH)
• Specificity: Detects Carboxymethyl Lysine adducts on proteins in Western blots. In Western blots, no cross-reactivity with carboxyethyl lysine adducts is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carboxymethyl Lysine Antibody (318003)

• Carboxymethyl Lysine
• CML

Background

N epsilon -carboxymethyl lysine (CML) is formed by the non-enzymatic Schiff-base reaction of glucose with proteins, followed by an Amadori rearrangement and oxidation that leaves only a carboxymethyl group attached to the lysine. The levels of CML adducts accumulate over time and have been used as an indicator of both serum glucose levels and oxidative protein damage. Elevated serum CML-modified proteins have been associated with diabetes and may contribute to diabetic retinopathy, nephropathy and angiopathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carboxymethyl Lysine Antibody (MAB3247)(16)

Publications using MAB3247

• Pino-Ramos, LL;Farias, DR;Olivares-Caro, L;Mitsi, C;Mardones, C;Echeverria, J;Avila, F;Gutierrez, M; Chilean papaya (Vasconcellea pubescens A. DC.) residues as a source of bioactive compounds: Chemical composition, antioxidant capacity, and antiglycation effects Heliyon 2024-10-15 [PMID: 39435094] (Western Blot, Plant)
• Rossi, GR;Jensen, A;Ng, S;Yin, Z;Li, A;Misra, A;Von Hoff, DD;Gruber, L;Gruber, M;Han, H; Advanced glycation end product (AGE) targeting antibody SIWA318H is efficacious in preclinical models for pancreatic cancer Scientific reports 2023-10-07 [PMID: 37805542] (ELISA Control, N/A)
• Susarla, G;Kataria, P;Kundu, A;D'Silva, P; Saccharomyces cerevisiae DJ-1 paralogs maintain genome integrity through glycation repair of nucleic acids and proteins eLife 2023-08-07 [PMID: 37548361] (Western Blot, Yeast)
• TT Lucy, ANM Mamun-Or-R, M Yagi, Y Yonei Serial Passaging of RAW 264.7 Cells Modulates Intracellular AGE Formation and Downregulates RANKL-Induced In Vitro Osteoclastogenesis International Journal of Molecular Sciences, 2022-02-21;23(4):. 2022-02-21 [PMID: 35216486] (Western Blot, Mouse)
• HH Dieguez, JS Calanni, HE Romeo, A Alaimo, MF González F, A Iaquinandi, MS Chianelli, MI Keller Sar, PH Sande, RE Rosenstein, D Dorfman Enriched environment and visual stimuli protect the retinal pigment epithelium and photoreceptors in a mouse model of non-exudative age-related macular degeneration Cell Death & Disease, 2021-12-04;12(12):1128. 2021-12-04 [PMID: 34864827] (IHC, Mouse)
• L Senatus, R López-Díez, L Egaña-Gorr, J Liu, J Hu, G Daffu, Q Li, K Rahman, Y Vengrenyuk, TJ Barrett, MZ Dewan, L Guo, D Fuller, AV Finn, R Virmani, H Li, RA Friedman, EA Fisher, R Ramasamy, AM Schmidt RAGE impairs murine diabetic atherosclerosis regression and implicates IRF7 in macrophage inflammation and cholesterol metabolism JCI Insight, 2020-07-09;5(13):. 2020-07-09 [PMID: 32641587] (Western Blot, Mouse)
• R Mastrocola, I Ferrocino, E Liberto, F Chiazza, AS Cento, D Collotta, G Querio, D Nigro, V Bitonto, JC Cutrin, K Rantsiou, M Durante, E Masini, M Aragno, C Cordero, L Cocolin, M Collino Fructose liquid and solid formulations differently affect gut integrity, microbiota composition and related liver toxicity: a comparative in vivo study J. Nutr. Biochem., 2018-02-13;55(0):185-199. 2018-02-13 [PMID: 29539590] (IHC-Fr, Western Blot, Mouse)
• E Benetti, R Mastrocola, F Chiazza, D Nigro, G D'Antona, V Bordano, R Fantozzi, M Aragno, M Collino, MA Minetto Effects of vitamin D on insulin resistance and myosteatosis in diet-induced obese mice PLoS ONE, 2018-01-17;13(1):e0189707. 1/17/2018 [PMID: 29342166]
• F Ávila, G Schmeda-Hi, E Silva The Major Chromophore Arising from Glucose Degradation and Oxidative Stress Occurrence during Lens Proteins Glycation Induced by Glucose Molecules, 2017-12-22;23(1):. 2017-12-22 [PMID: 29271874] (Western Blot, Bovine)
• S Jang, DM Kwon, K Kwon, C Park Generation and characterization of mouse knockout for glyoxalase 1 Biochem. Biophys. Res. Commun., 2017-06-13;0(0):. 2017-06-13 [PMID: 28623132] (Western Blot, Mouse)
• Mastrocola , Raffaell, Collino , Massimo, Nigro , Debora, Chiazza , Fausto, D'Antona , Giuseppe, Aragno , Manuela, Minetto , Marco A Accumulation of advanced glycation end-products and activation of the SCAP/SREBP Lipogenetic pathway occur in diet-induced obese mouse skeletal muscle. PLoS ONE, 2015-03-09;10(3):e0119587. 2015-03-09 [PMID: 25750996] (Western Blot, Mouse)
• Talaei , Fatemeh, Van Praag , Veroniek, Shishavan , Mahdi H, Landheer , Sjoerd W, Buikema , Henk, Henning , Robert H Increased protein aggregation in Zucker diabetic fatty rat brain: identification of key mechanistic targets and the therapeutic application of hydrogen sulfide. BMC Cell Biol, 2014-01-06;15(0):1. 2014-01-06 [PMID: 24393531] (Western Blot, Mouse)
• Chen , Chih-Yu, Abell , Allison, Moon , Yang Soo, Kim , Kee-Hong An advanced glycation end product (AGE)-receptor for AGEs (RAGE) axis restores adipogenic potential of senescent preadipocytes through modulation of p53 protein function. J Biol Chem, 2012-11-13;287(53):44498-507. 2012-11-13 [PMID: 23150674] (Western Blot, Mouse)
• James P. Chow, Dan T. Simionescu, Harleigh Warner, Bo Wang, Sourav S. Patnaik, Jun Liao et al. Mitigation of diabetes-related complications in implanted collagen and elastin scaffolds using matrix-binding polyphenol Biomaterials 1/1/2013 [PMID: 23103157]
• Jiaqian Ni, Xianglin Yuan, Jiayin Gu, Xiuzhen Yue, Xiaorong Gu, Ram H. Nagaraj et al. Plasma Protein Pentosidine and Carboxymethyllysine, Biomarkers for Age-related Macular Degeneration* Molecular & Cellular Proteomics 8/1/2009 [PMID: 19435712]
• Shapiro BP, Owan TE, Mohammed SF, Meyer DM, Mills LD, Schalkwijk CG, Redfield MM Advanced glycation end products accumulate in vascular smooth muscle and modify vascular but not ventricular properties in elderly hypertensive canines. Circulation, 2008-08-18;118(10):1002-10. 2008-08-18 [PMID: 18711013] (Western Blot, Canine)

Review for Carboxymethyl Lysine Antibody (MAB3247) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Patricia Carulla Martí IHC-P Human 05/30/2024

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human skin
• Species: Human
• Lot: WWZ0322041

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***