Carnosine Dipeptidase 1/CNDP1 Antibody [Biotin]

Identification and validation of CNPD1 reduction in CC.Detected protein levels are shown for A. the screen using Sandwich Bead Arrays (SBA) and B. validation by Sandwich Immunoassays (SIA) and expressed as mean intensity fluorescence (MIF). C. There was an excellent correlation between results obtained by SBA and SIA. CNPD1 levels measured by SIA were not influenced by D. age or E. gender. F. Distribution of circulating CNDP1 concentrations determined by SIA in Cohort 2. WS = Weight stable, CC = Cancer cachexia. P- and rho-values are given. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0123566), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Carnosine Dipeptidase 1/CNDP1 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Carnosine Dipeptidase 1/CNDP1
  Pro28-His507
  Accession # Q96KN2
• Specificity: Detects human Carnosine Dipeptidase 1/CNDP1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CNDP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carnosine Dipeptidase 1/CNDP1 Antibody [Biotin]

• beta-Ala-His dipeptidase
• Carnosinase 1
• carnosine dipeptidase 1 (metallopeptidase M20 family)
• Carnosine Dipeptidase 1
• CN1MGC102737
• CNDP dipeptidase 1
• CNDP1
• CPGL2
• CPGL2MGC142072
• EC 3.4.13.20
• Glutamate carboxypeptidase-like protein 2
• HsT2308
• MGC10825
• Serum carnosinase

Background

The human CNDP1 gene encodes carnosine dipeptidase 1, a member of the M20 family of metalloproteases (1, 2). Also known as X-His dipeptidase, glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), CNDP1 is a secreted dipeptidase with a narrow specificity for Xaa-His dipeptides including those with Xaa = beta -Ala (carnosine) and Xaa = gamma -aminobutyric acid (homocarnosine), two naturally occurring dipeptides with potential neuroprotective and neurotransmitter fucntions in the brain. In comparison, a closely related protein known as CNDP2, CPGL or CN2, is a cytosolic nonspecific dipeptidase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carnosine Dipeptidase 1/CNDP1 Antibody (BAF2489)(1)

Publication using BAF2489

• Arner P, Henjes F, Schwenk J, Darmanis S, Dahlman I, Iresjo B, Naredi P, Agustsson T, Lundholm K, Nilsson P, Ryden M Circulating carnosine dipeptidase 1 associates with weight loss and poor prognosis in gastrointestinal cancer. PLoS ONE, 2015-04-21;10(4):e0123566. 2015-04-21 [PMID: 25898255] (ELISA Development (Detection), Human)

Bioinformatics

• Gene Symbol: CNDP1
• Uniprot:
  • Q96KN2()