Caspase-1 Antibody

Western Blot: Caspase-1 Antibody [NBP1-45433] - Exendin-4 inhibits diabetic cardiomyocyte pyroptosis: (f) Western blot of pyroptotic proteins. The primary cardiomyocytes were incubated in the absence (CON) or presence of 40mM glucose (HG) in combination with Exendin-4 (25nM) (HG+EXE). Image collected and cropped by CiteAb from the following publication (ncbi.nlm.nih.gov/pmc/articles/PMC6925927/), licensed under a CC-BY license.

Western Blot: Caspase-1 Antibody [NBP1-45433] - Analysis of Caspase-1 expression in Rat liver tissue lysate.

Product Discontinued

Caspase-1 Antibody Summary

• Immunogen: Caspase-1 Antibody was developed against a synthetic peptide surrounding amino acid 132 of human caspase-1 (NP_150636).
• Specificity: Caspase-1 Antibody recognizes the 45 kDa procaspase-1 and the 20 kDa cleaved product.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 - 20 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 4 - 8 ug/ml
  • Knockdown Validated
  • Western Blot 0.5 - 4 ug/ml
• Application Notes: Knockdown validation (PMID: 31886288). Use in Immunohistochemistry reported in scientific literature (PMID: 32201254).
• Control:

  Jurkat Staurosporine Treated / Untreated Cell Lysate

Reactivity Notes

E. coli reactivity reported in scientific literature (PMID: 31551961).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 0.5 mM EDTA, 0.5% BSA, 30% glycerol
• Preservative: 0.03% Proclin 300
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for Caspase-1 Antibody

• CASP1 nirs variant 1
• CASP1
• CASP-1
• caspase 1, apoptosis-related cysteine peptidase
• Caspase-1 subunit p10
• Caspase-1 subunit p20
• Caspase1
• Caspase-1
• EC 3.4.22.36
• EC:3.4.22.36
• ICE
• IL-1 beta-converting enzyme
• IL1BC
• IL-1BC
• IL1BCE
• IL1B-convertase
• interleukin 1, beta, convertase
• interleukin 1-B converting enzyme
• Interleukin-1 beta convertase
• Interleukin-1 beta-converting enzyme
• p45

Background

Caspase-1, also known as interleukin-1-beta (IL-1beta) -converting enzyme (ICE), is a member of the cysteine-aspartic protease (caspase) family (1). The caspase family are aspartic proteolytic enzymes that play a key role in apoptotic cell death and inflammation (1). Caspase-1 is synthesized as an inactive proenzyme with a theoretical molecular weight of approximately 45 kDa and is 404 amino acids (aa) in length. The caspase-1 protein structure contains an N-terminal CARD (caspase recruitment domain), a large 20kDa P20 subunit (aa 120-297), and a small 10kDa P10 subunit (aa 317-404) (1, 2). The proenzyme undergoes cleavage at aspartic acid (Asp) residues to produce the active caspase-1 enzyme which exists as a heterotetramer (homodimer of the P20 and P10 heterodimers) (2). Inflammasome complexes sense various microbial and endogenous signals, including ligand-receptor interaction and growth factor deprivation, which results in the processing of pro-caspase to active caspase-1 (3, 4).The active caspase-1 is responsible for the cleavage and maturation of pro-inflammatory cytokines IL-1beta and IL-18, and promotes an inflammatory form of cell death induced by bacterial pathogens called pyroptosis (3, 4). Mature IL-1beta has a role in the immune reaction and recruiting inflammatory cells to the site of infection while IL-18 plays a role in interferon-gamma production and promotes cytolytic activity in natural killer cells (4).

Given the role of IL-1beta in inflammation, it makes sense that many diseases and pathologies have been associated with dysregulation of caspase-1 activation and the inflammasome (3, 4). The inflammasome is a multiprotein complex comprised of Nod-like receptor (NLR) family members and the adapter ASC (apoptosis-associated speck-like protein containing a CARD) which are crucial for capase-1 activation (3-5). For instance, the neuronal apoptosis inhibitory protein (NAIP)/NLRC4 inflammasome has been associated with colorectal cancer, breast cancer, and glioma pathogenesis (5). Caspase-1 activation and mutations in the inflammasome have also been linked to Chron's disease and Alzheimer's disease (4). In addition to immune and inflammatory related disorder, the inflammasome has been linked to metabolic and obesity related disorders including diabetes and cardiovascular disease (6). Finally, caspase-1 deficient mice exhibit enhanced epithelial cell proliferation in the colon, increased tumor formation, and reduced apoptosis (1). A more thorough understanding of the inflammasome-caspase-1 signaling pathway will be important for understanding disease pathology and potential therapeutic development.

Alternative names for caspase-1 includes CASP1, CASP1 nirs variant 1, EC 3.4.22.36, ICE, IL-1 beta-converting enzyme, IL1BC, IL1BCE, IL1B-converstase, interleukin-1 beta convertase, and p45.

References

1. Shalini, S., Dorstyn, L., Dawar, S., & Kumar, S. (2015). Old, new and emerging functions of caspases. Cell death and differentiation. https://doi.org/10.1038/cdd.2014.216

2. Chang, H. Y., & Yang, X. (2000). Proteases for cell suicide: functions and regulation of caspases. Microbiology and molecular biology reviews: MMBR. https://doi.org/10.1128/mmbr.64.4.821-846.2000

3. Vanaja, S. K., Rathinam, V. A., & Fitzgerald, K. A. (2015). Mechanisms of inflammasome activation: recent advances and novel insights. Trends in cell biology. https://doi.org/10.1016/j.tcb.2014.12.009

4. Franchi, L., Eigenbrod, T., Munoz-Planillo, R., & Nunez, G. (2009). The inflammasome: a caspase-1-activation platform that regulates immune responses and disease pathogenesis. Nature immunology. https://doi.org/10.1038/ni.1703

5. Kay, C., Wang, R., Kirkby, M., & Man, S. M. (2020). Molecular mechanisms activating the NAIP-NLRC4 inflammasome: Implications in infectious disease, autoinflammation, and cancer. Immunological reviews. https://doi.org/10.1111/imr.12906

6. Pham, D., Park, P. (2020). Recent insights on modulation of inflammasomes by adipokines: a critical event for the pathogenesis of obesity and metabolism-associated diseases. Archives of Pharmacal Research. https://doi.org/10.1007/s12272-020-01274-7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-1 Antibody (NBP1-45433)(48)

Publications using NBP1-45433

• Li Z, Dai R, Chen M et al. p55? degrades RIP3 via MG53 to suppress ischemia-induced myocardial necroptosis and mediates cardioprotection of preconditioning Cardiovascular research 2023-08-01 [PMID: 37527538]
• Muñoz-García R, Sánchez-Hidalgo M, Alcarranza M et al. Effects of Dietary Oleacein Treatment on Endothelial Dysfunction and Lupus Nephritis in Balb/C Pristane-Induced Mice Antioxidants (Basel, Switzerland) 2023-06-19 [PMID: 37372034] (WB, Mouse)
• Wang G, Yin W, Shin H Et al. Neuronal accumulation of peroxidated lipids promotes demyelination and neurodegeneration through the activation of the microglial NLRP3 inflammasome Nature Aging 2021-11-01 [PMID: 37118341]
• Wang Y, Sadike D, Huang B et al. Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-?B pathway in LPC-induced demyelination Journal of neuroinflammation 2023-02-18 [PMID: 36803990] (IHC, Mouse)
• Chen C, Wang W, Raymond M et al. Genetic Knockout of Fatty Acid Amide Hydrolase Ameliorates Cisplatin-Induced Nephropathy in Mice Molecular pharmacology 2023-04-01 [PMID: 36702548] (WB, Mouse)
• Singh G, Storey KB TXNIP shuttling - a key molecular link in regulating inflammation and mitochondrial dysfunction in freeze tolerant wood frogs Gene 2023-01-07 [PMID: 36627089] (WB, Amphibian)
• Montoya T, SAnchez-Hidalgo M, CastejOn ML et al. Oleocanthal Supplemented Diet Improves Renal Damage And Endothelial Dysfunction In Pristane-Induced Systemic Lupus Erythematosus In Mice Food Research International 2022-11-01 [PMID: 36596095] (Mouse)
• Lu Y PROATHEROGENIC LIPIDS INCREASE CASPASE-1 NUCLEAR LOCALIZATION IN HUMAN AORTIC ENDOTHELIAL CELLS Thesis 2020-01-01
• Singh G Role of Glucose-induced Transcription Factor Signalling and Mitochondrial Epigenetics in Stress Tolerant Wood Frog, Rana sylvatica Thesis 2022-12-16 (Western Blot, Amphibian)
  
  Details:
  Wood Frog
• McElwain CJ, Musumeci A, Manna S et al. L-ergothioneine reduces mitochondrial-driven NLRP3 activation in gestational diabetes mellitus Journal of reproductive immunology 2023-11-24 [PMID: 38029485] (WB, Human)
• Hwang S, Kim SH, Yoo KH et al. Exogenous 8-hydroxydeoxyguanosine attenuates doxorubicin-induced cardiotoxicity by decreasing pyroptosis in H9c2 cardiomyocytes BMC molecular and cell biology 2022-12-14 [PMID: 36517746] (WB, Mouse)
• Xiao Q, Kang Z, Liu C et al. Panax notoginseng Saponins Attenuate Cerebral Ischemia-Reperfusion Injury via Mitophagy-Induced Inhibition of NLRP3 Inflammasome in Rats Frontiers in Bioscience-Landmark 2022-11-03 [PMID: 36472098] (WB, Rat)
  
  Details:
  Dilution used 1:500
• Babaei F, Mirzababaei M, Dargahi L et al. Preventive Effect of Saccharomyces boulardii on Memory Impairment Induced by Lipopolysaccharide in Rats ACS chemical neuroscience 2022-11-01 [PMID: 36318666]
• Eggelbusch M, Shi A, Broeksma BC et al. The NLRP3 inflammasome contributes to inflammation-induced morphological and metabolic alterations in skeletal muscle Journal of cachexia, sarcopenia and muscle 2022-08-17 [PMID: 35978267] (WB, Human)
• Ke Q, Shi C, Lv Y et al. SGLT2 inhibitor counteracts NLRP3 inflammasome via tubular metabolite itaconate in fibrosis kidney FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2022-01-01 [PMID: 34918381] (WB, Mouse)
• Li M, Lu H, Wang X Et al. Pyruvate kinase M2 (PKM2) interacts with activating transcription factor 2 (ATF2) to bridge glycolysis and pyroptosis in microglia Molecular immunology 2021-11-17 [PMID: 34798593] (WB, Mouse)
• Fan X, Zhang J, Dai Y Et al. Blockage of P2X7R suppresses Th1/Th17-mediated immune responses and corneal allograft rejection via inhibiting NLRP3 inflammasome activation Experimental eye research 2021-10-14 [PMID: 34656546] (WB, ICC/IF, Mouse)
• Lu Y, Nanayakkara G, Sun Y et al. Procaspase-1 patrolled to the nucleus of proatherogenic lipid LPC-activated human aortic endothelial cells induces ROS promoter CYP1B1 and strong inflammation Redox biology 2021-09-27 [PMID: 34598017] (WB, Human)
• ZHOU X, Yin K, Jiang W et al. Jiangzhi Ligan Decoction Alleviated Nonalcoholic Fatty Liver Disease Induced by High-Fat-Diet in Rats via GSDMD-mediated Canonical/Non-canonical Pyroptosis Pathway Dis Markers 2021-07-09 [PMID: 34239622]
• Liu X, Liu H, Lu X, Zhao S N-acetylcysteine alleviates ocular surface damage in STZ-induced diabetic mice by inhibiting the ROS/NLRP3/Caspase-1/IL-1 beta signaling pathway Experimental eye research 2021-06-05 [PMID: 34102208]
• Zhou K, Enkhjargal B, Mo J et al. Dihydrolipoic acid enhances autophagy and alleviates neurological deficits after subarachnoid hemorrhage in rats Experimental neurology 2021-05-08 [PMID: 33974879]
• Liang H, Matei N, Devin W et al. TGR5 activation attenuates neuroinflammation via Pellino3 inhibition of Caspase-8/NLRP3 after middle cerebral artery occlusion in rats Research Square 2020-12-01 [PMID: 33531049] (WB, Rat)
• Li L, Zou Y, Liu B et al. Contribution of the P2X4 Receptor in Rat Hippocampus to the Comorbidity of Chronic Pain and Depression ACS chemical neuroscience 2020-12-16 [PMID: 33284579]
• Jiang S, Zhang H, Li X et al. Vitamin D/VDR attenuate cisplatin-induced AKI by down-regulating NLRP3/Caspase-1/GSDMD pyroptosis pathway J Steroid Biochem Mol Biol 2020-11-28 [PMID: 33259938] (WB, Mouse)
• Zhang Q, Wang J, Huang F et al. Leptin induces NAFLD progression through infiltrated CD8+ T lymphocytes mediating pyroptotic-like cell death of hepatocytes and macrophages Dig Liver Dis 2020-11-07 [PMID: 33172809] (WB, Rat)
• Lim JR, Lee HJ, Jung YH et al. Ethanol-activated CaMKII signaling induces neuronal apoptosis through Drp1-mediated excessive mitochondrial fission and JNK1-dependent NLRP3 inflammasome activation Cell Commun. Signal 2020-08-12 [PMID: 32787872] (WB, Human)
• Wang C, Jia Q, Sun C, Jing C Calcium sensing receptor contribute to early brain injury through the CaMKII/NLRP3 pathway after subarachnoid hemorrhage in mice Biochem. Biophys. Res. Commun. 2020-10-01 [PMID: 32768195] (WB, Mouse)
• Verma V, Kumar P, Gupta S et al. Alpha-Hemolysin of uropathogenic E. coli regulates NLRP3 inflammasome activation and mitochondrial dysfunction in THP-1 macrophages Sci Rep 2020-07-28 [PMID: 32724079] (WB, Human)
• Shi Y, Shi X, Liang J et al. Aggravated MRSA pneumonia secondary to influenza A virus infection is derived from decreased expression of IL-1 beta J. Med. Virol. 2020-07-22 [PMID: 32697385] (WB, Mouse)
• Saber S, Abd El-Kader EM, Sharaf H et al. Celastrol augments sensitivity of NLRP3 to CP-456773 by modulating HSP-90 and inducing autophagy in dextran sodium sulphate-induced colitis in rats Toxicol. Appl. Pharmacol. 2020-05-26 [PMID: 32470352] (IF/IHC, Rat)
• Liu G, Baird AW, Parsons MJ et al. Platelet activating factor receptor acts to limit colitis-induced liver inflammation FASEB J. 2020-04-15 [PMID: 32293760] (WB, Mouse)
• Kim H, Seo Js, Lee Sy et Al. AIM2 inflammasome contributes to brain injury and chronic post-stroke cognitive impairment in mice Brain Behav. Immun. 2020-03-19 [PMID: 32201254] (IF/IHC, WB, Mouse)
• Yu J, Wang WN, Matei N et al. Ezetimibe Attenuates Oxidative Stress and Neuroinflammation via the AMPK/Nrf2/TXNIP Pathway after MCAO in Rats Oxid Med Cell Longev 2020-01-04 [PMID: 31998437] (WB, Rat)
• Wei H, Bu R, Yang Q Exendin-4 Protects against Hyperglycemia-Induced Cardiomyocyte Pyroptosis via the AMPK-TXNIP Pathway J Diabetes Res 2019-12-09 [PMID: 31886288] (WB, KD, Mouse)
• Verma V, Gupta S, Kumar P et al. Involvement of NLRP3 and NLRC4 Inflammasome in Uropathogenic E. coli Mediated Urinary Tract Infections Front Microbiol 2019-09-26 [PMID: 31551961] (WB, E. coli)
• Zhao S, Zhou Y, Fan Y et al. Involvement of P2X4 receptor in gp120-induced pyroptosis in dorsal root ganglia J. Neurochem. 2019-08-16 [PMID: 31418825]
• Liu B, Mao X, Huang D et al. Novel role of NLRP3-inflammasome in regulation of lipogenesis in fasting-induced hepatic steatosis Diabetes Metab Syndr Obes 2019-05-27 [PMID: 31239738] (WB, Mouse)
• Liu, G;Mateer, SW;Hsu, A;Goggins, BJ;Tay, H;Mathe, A;Fan, K;Neal, R;Bruce, J;Burns, G;Minahan, K;Maltby, S;Fricker, M;Foster, PS;Wark, PAB;Hansbro, PM;Keely, S; Platelet activating factor receptor regulates colitis-induced pulmonary inflammation through the NLRP3 inflammasome Mucosal Immunol 2019-04-11 [PMID: 30976089] (WB, Mouse)
• Lopes de Oliveira GA, Alarcon de la Lastra C, Rosillo Ma et al. Preventive effect of bergenin against the development of TNBS-induced acute colitis in rats is associated with inflammatory mediators inhibition and NLRP3/ASC inflammasome signaling pathways. Chem. Biol. Interact. 2018-10-23 [PMID: 30365937] (WB, Rat)
• Che C, Li C, Lin J et al. Wnt5a contributes to dectin-1 and LOX-1 induced host inflammatory response signature in Aspergillus fumigatus keratitis. Cell. Signal. 2018-12-01 [PMID: 30172652] (WB, Mouse, Human)
• Chen N, Ou Z, Zhang W et al. Cathepsin B regulates non-canonical NLRP3 inflammasome pathway by modulating activation of caspase-11 in Kupffer cells Cell Prolif. 2018-08-07 [PMID: 30084208] (WB, Human)
• Mandal P, Feng Y, LYons JD et al. Caspase-8 Collaborates with Caspase-11 to Drive Tissue Damage and Execution of Endotoxic Shock. Immunity. 2018-07-17 [PMID: 30021146] (Mouse)
• Zhou K, Enkhjargal B, Xie Z et al. Dihydrolipoic Acid Inhibits Lysosomal Rupture and NLRP3 Through Lysosome-Associated Membrane Protein-1/Calcium/Calmodulin-Dependent Protein Kinase II/TAK1 Pathways After Subarachnoid Hemorrhage in Rat Stroke 2018-01-01 [PMID: 29273596] (WB, Rat)
• Hu Q, Zhang T, Yi L et al. Dihydromyricetin inhibits NLRP3 inflammasome-dependent pyroptosis by activating the Nrf2 signaling pathway in vascular endothelial cells. Biofactors 2017-11-29 [PMID: 29193391] (Human)
• Chen ML, Zhu XH, Ran L et al. Trimethylamine-N-Oxide Induces Vascular Inflammation by Activating the NLRP3 Inflammasome Through the SIRT3-SOD2-mtROS Signaling Pathway J Am Heart Assoc 2017-09-04 [PMID: 28871042] (WB, Human)
• Lee HI, Lee SW, Kim NG et al. Low-level light emitting diode (LED) therapy suppresses inflammasome-mediated brain damage in experimental ischemic stroke. J Biophotonics 2017-02-06 [PMID: 28164443] (WB, Mouse)
• Lin C, Chao H, Li Z et al. Omega-3 fatty acids regulate NLRP3 inflammasome activation and prevent behavior deficits after traumatic brain injury Exp. Neurol 2017-01-08 [PMID: 28077335]
• Draganov D, Gopalakrishna-Pillai S, Chen YR et al. Modulation of P2X4/P2X7/Pannexin-1 sensitivity to extracellular ATP via Ivermectin induces a non-apoptotic and inflammatory form of cancer cell death. Sci Rep. 2015-11-10 [PMID: 26552848] (WB, Human)

Reviews for Caspase-1 Antibody (NBP1-45433) (2)

Average Rating: 3.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer WB Human 02/28/2018

Summary

• Application: Western Blot
• Sample Tested: THP-1 cell lysate
• Species: Human
• Lot: 3L20L30190

reviewed by: Chunhui Sun WB Human 07/21/2015

Summary

• Application: Western Blot
• Sample Tested: Cell lysate
• Species: Human

FAQs for Caspase-1 Antibody (NBP1-45433). (Showing 1 - 1 of 1 FAQs).

1. Do you have any Caspase-1 antibodies that recognize bacterial species?
   • NBP1-45433 has been validated in E. coli. If you would like to use any of our Caspase-1 antibodies on a species that's not listed as a validated species, you can review our Innovator's Reward Program.

Bioinformatics

• Gene Symbol: CASP1
• Entrez:
  • 834(Human)
• Uniprot:
  • NP_150636(Human)