Mouse Caspase-1 ELISA Kit (Colorimetric)

ELISA: Mouse Caspase-1 ELISA Kit (Colorimetric) [NBP2-75014] - Samples were spiked with high concentrations of Mouse Caspase-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Caspase-1 ELISA Kit (Colorimetric) [NBP2-75014] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL

Mouse Caspase-1 ELISA Kit (Colorimetric) Summary

• Specificity: Mouse Caspase-1 ELISA Kit (Colorimetric) recognizes Mouse CASP1 in samples. No significant cross-reactivity or interference between Mouse CASP1 and analogues was observed.
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.94%
• Intra-Assay: CV% < 5.54%
• Spike Recovery: 87-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CASP1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Caspase-1 ELISA Kit (Colorimetric)

• CASP1 nirs variant 1
• CASP1
• CASP-1
• caspase 1, apoptosis-related cysteine peptidase
• Caspase-1 subunit p10
• Caspase-1 subunit p20
• Caspase1
• Caspase-1
• EC 3.4.22.36
• EC:3.4.22.36
• ICE
• IL-1 beta-converting enzyme
• IL1BC
• IL-1BC
• IL1BCE
• IL1B-convertase
• interleukin 1, beta, convertase
• interleukin 1-B converting enzyme
• Interleukin-1 beta convertase
• Interleukin-1 beta-converting enzyme
• p45

Background

Caspase-1, also known as interleukin-1-beta (IL-1beta) -converting enzyme (ICE), is a member of the cysteine-aspartic protease (caspase) family (1). The caspase family are aspartic proteolytic enzymes that play a key role in apoptotic cell death and inflammation (1). Caspase-1 is synthesized as an inactive proenzyme with a theoretical molecular weight of approximately 45 kDa and is 404 amino acids (aa) in length. The caspase-1 protein structure contains an N-terminal CARD (caspase recruitment domain), a large 20kDa P20 subunit (aa 120-297), and a small 10kDa P10 subunit (aa 317-404) (1, 2). The proenzyme undergoes cleavage at aspartic acid (Asp) residues to produce the active caspase-1 enzyme which exists as a heterotetramer (homodimer of the P20 and P10 heterodimers) (2). Inflammasome complexes sense various microbial and endogenous signals, including ligand-receptor interaction and growth factor deprivation, which results in the processing of pro-caspase to active caspase-1 (3, 4).The active caspase-1 is responsible for the cleavage and maturation of pro-inflammatory cytokines IL-1beta and IL-18, and promotes an inflammatory form of cell death induced by bacterial pathogens called pyroptosis (3, 4). Mature IL-1beta has a role in the immune reaction and recruiting inflammatory cells to the site of infection while IL-18 plays a role in interferon-gamma production and promotes cytolytic activity in natural killer cells (4).

Given the role of IL-1beta in inflammation, it makes sense that many diseases and pathologies have been associated with dysregulation of caspase-1 activation and the inflammasome (3, 4). The inflammasome is a multiprotein complex comprised of Nod-like receptor (NLR) family members and the adapter ASC (apoptosis-associated speck-like protein containing a CARD) which are crucial for capase-1 activation (3-5). For instance, the neuronal apoptosis inhibitory protein (NAIP)/NLRC4 inflammasome has been associated with colorectal cancer, breast cancer, and glioma pathogenesis (5). Caspase-1 activation and mutations in the inflammasome have also been linked to Chron's disease and Alzheimer's disease (4). In addition to immune and inflammatory related disorder, the inflammasome has been linked to metabolic and obesity related disorders including diabetes and cardiovascular disease (6). Finally, caspase-1 deficient mice exhibit enhanced epithelial cell proliferation in the colon, increased tumor formation, and reduced apoptosis (1). A more thorough understanding of the inflammasome-caspase-1 signaling pathway will be important for understanding disease pathology and potential therapeutic development.

Alternative names for caspase-1 includes CASP1, CASP1 nirs variant 1, EC 3.4.22.36, ICE, IL-1 beta-converting enzyme, IL1BC, IL1BCE, IL1B-converstase, interleukin-1 beta convertase, and p45.

References

1. Shalini, S., Dorstyn, L., Dawar, S., & Kumar, S. (2015). Old, new and emerging functions of caspases. Cell death and differentiation. https://doi.org/10.1038/cdd.2014.216

2. Chang, H. Y., & Yang, X. (2000). Proteases for cell suicide: functions and regulation of caspases. Microbiology and molecular biology reviews: MMBR. https://doi.org/10.1128/mmbr.64.4.821-846.2000

3. Vanaja, S. K., Rathinam, V. A., & Fitzgerald, K. A. (2015). Mechanisms of inflammasome activation: recent advances and novel insights. Trends in cell biology. https://doi.org/10.1016/j.tcb.2014.12.009

4. Franchi, L., Eigenbrod, T., Munoz-Planillo, R., & Nunez, G. (2009). The inflammasome: a caspase-1-activation platform that regulates immune responses and disease pathogenesis. Nature immunology. https://doi.org/10.1038/ni.1703

5. Kay, C., Wang, R., Kirkby, M., & Man, S. M. (2020). Molecular mechanisms activating the NAIP-NLRC4 inflammasome: Implications in infectious disease, autoinflammation, and cancer. Immunological reviews. https://doi.org/10.1111/imr.12906

6. Pham, D., Park, P. (2020). Recent insights on modulation of inflammasomes by adipokines: a critical event for the pathogenesis of obesity and metabolism-associated diseases. Archives of Pharmacal Research. https://doi.org/10.1007/s12272-020-01274-7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Caspase-1 ELISA Kit (NBP2-75014)(6)

Publications using NBP2-75014

• Gravina G, Ardalan M, Chumak T et al. Transcriptome network analysis links perinatal Staphylococcus epidermidis infection to microglia reprogramming in the immature hippocampus Glia 2023-05-29 [PMID: 37246946] (ELISA)
• Park Y, Dodantenna N, Kim T et al. MARCH5-dependent NLRP3 ubiquitination is an essential step for NEK7 docking on the mitochondria bioRxiv 2023-01-12 (ELISA)
• Kang-Gu Lee, Bong-Ki Hong, Saseong Lee, Naeun Lee, Seung-Whan Kim, Donghyun Kim, Wan-Uk Kim Nuclear receptor coactivator 6 is a critical regulator of NLRP3 inflammasome activation and gouty arthritis Cellular and Molecular Immunology 2024-01-10 [PMID: 38195836] (ELISA)
• S Singh, IA Shaikh, SS More, MH Mahnashi, HM Almohaimee, M El-Sherbin, MM Ghoneim, A Umar, HK Soni, H Agrawal, BA Mannasaheb, AA Khan, UM Muddapur, SMS Iqubal Blockage of KHSRP-NLRP3 by MCC950 Can Reverse the Effect of Manganese-Induced Neuroinflammation in N2a Cells and Rat Brain International Journal of Molecular Sciences, 2022-10-30;23(21):. 2022-10-30 [PMID: 36362011] (Mouse, ELISA)
• Ammar RA, Mohamed AF, Kamal MM et al. Neuroprotective effect of liraglutide in an experimental mouse model of multiple sclerosis: role of AMPK/SIRT1 signaling and NLRP3 inflammasome Inflammopharmacology 2022-04-01 [PMID: 35364735] (ELISA)
• Lee Jin-Seok, Kim Won-Young, Jeon Yoo-Jin et al. Antidepressant-Like Activity of Myelophil via Attenuation of Microglial-Mediated Neuroinflammation in Mice Undergoing Unpredictable Chronic Mild Stress. Frontiers in Pharmacology 2019-06-13 [PMID: 31263417] (ELISA, Mouse)

Bioinformatics

• Gene Symbol: CASP1