Cathepsin B Antibody (155714) - Pro form

• Reactivity: Hu
• Applications: IP, ELISA(Cap)
• Clone: 155714
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cathepsin B Antibody (155714) - Pro form Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Cathepsin B
  Arg18-Ile339
  Accession # P07858
• Specificity: Detects human Cathepsin B. Does not cross-react with recombinant human Cathepsin A, C, D, E, L, S, V, X/Z/P, SA, or recombinant mouse Cathepsin B.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTSB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • ELISA Standard (Matched Pair)
  • Immunoprecipitation 25 ug/mL
• Application Notes: ELISA Detection: Human Pro-Cathepsin B Biotinylated Antibody (Catalog number BAM21761)
  Standard: Recombinant Human Cathepsin B (Catalog number 953-CY)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin B Antibody (155714) - Pro form

• APP secretase
• APPS
• Cathepsin B
• Cathepsin B1
• CPSBamyloid precursor protein secretase
• CTSB
• cysteine protease
• EC 3.4.22
• EC 3.4.22.1

Background

Cathepsin B is the first described member of the family of lysosomal cysteine proteases (1). Cathepsin B possesses both endopeptidase and exopeptidase activities, in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins, including pro and active caspases, prorenin and secretory leucoprotease inhibitor (SLPI) (2 - 4). Therefore, Cathepsin B may play a role in activation and inactivation of caspases, activation of renin and inactivation of SLPI, the key steps in apoptosis, angiotensin production, and progression of emphysema, respectively. Because of its increased levels and redistribution of the enzyme in human and animal tumors, Cathepsin B may also have role in invasion and metastasis (5).

In addition to lysosome, Cathepsin B can be secreted or associated with plasma membrane, cytoplasm, and nucleus. It is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme (1).

1. Mort, J.S. (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. (eds): Academic Press, San Diego, p. 1079.
2. Vancompernolle, K. et al. (1998) FEBS Lett. 438:150.
3. Jutras, I. and T.L. Reudelhuber (1999) FEBS Lett. 443:48.
4. Taggart, C.C. et al. (2001) J. Biol. Chem. 276:33345.
5. Bergquin, I.M. and B.F. Sloane (1996) Adv. Exp. Med. Biol. 389:281.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cathepsin B Antibody (MAB2176)(1)

Publication using MAB2176

• Lopez-Castejon G, Theaker J, Pelegrin P P2X(7) receptor-mediated release of cathepsins from macrophages is a cytokine-independent mechanism potentially involved in joint diseases. J. Immunol., 2010-07-16;185(4):2611-9. 2010-07-16 [PMID: 20639492] (Immunodepletion, Human)

Bioinformatics

• Gene Symbol: CTSB
• Uniprot:
  • P07858()