Cathepsin B Assay Kit (Fluorometric)

Product Discontinued

Cathepsin B Assay Kit (Fluorometric) Summary

• Description: Rapid and convenient fluorometric assay to measure Cathepsin B Activity within 1-2 hrs.
  
  Detection method: Fluorometer or fluorescence plate reader (Ex/Em = 400/505 nm)Applications: Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65300)
• Kit Type: Assay Kit (Fluorometric)
• Gene: CTSB

Applications/Dilutions

Publications

Read Publication using NBP2-54841.

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Cathepsin B Assay Kit (Fluorometric)

• APP secretase
• APPS
• Cathepsin B
• Cathepsin B1
• CPSBamyloid precursor protein secretase
• CTSB
• cysteine protease
• EC 3.4.22
• EC 3.4.22.1

Background

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations. The Cathepsin-B Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate sequence RR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate RR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Cathepsin B Kit (NBP2-54841)(1)

Publication using NBP2-54841

• Marchand B, Poulin MA, Lawson C et al. Gemcitabine promotes autophagy and lysosomal function through ERK- and TFEB-dependent mechanisms Cell death discovery 2023-02-06 [PMID: 36746928]

Bioinformatics

• Gene Symbol: CTSB