CCL4/MIP-1 beta Antibody (24006) [Allophycocyanin]

Human peripheral blood monocytes treated with LPS and Monensin for 5 hours were stained with Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCL4/MIP-1 beta APC-conjugated Monoclonal Antibody (Catalog # IC271A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 24006
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

CCL4/MIP-1 beta Antibody (24006) [Allophycocyanin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CCL4/MIP-1 beta
  Ala24-Asn92
  Accession # P13236
• Specificity: Detects human CCL4/MIP-1 beta in ELISAs and Western blots. In Western blots, this antibody shows less than 4% cross-reactivity with recombinant human (rh) CCL3/MIP-1 alpha and does not cross-react with recombinant mouse (rm) CCL3/MIP-1 alpha , rmCCL4/MIP-1 beta , rhCXCL8/IL-8, rhCCL5, rhCXCL1, rhCXCL2, or rhCXCL3.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCL4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCL4/MIP-1 beta Antibody (24006) [Allophycocyanin]

• ACT2
• ACT-2
• AT744.1
• C-C motif chemokine 4
• CCL4
• chemokine (C-C motif) ligand 4
• Exodus-3
• G-26 T-lymphocyte-secreted protein
• G-26
• HC21
• LAG1
• LAG-1
• Lymphocyte activation gene 1 protein
• Macrophage inflammatory protein 1-beta
• MIP1 beta
• MIP-1 beta
• MIP1B
• MIP1B1
• MIP-1-beta
• MIP-1-beta(1-69)
• PAT 744
• Protein H400
• SCYA2
• SCYA4
• secreted protein G-26
• SIS-gamma
• small inducible cytokine A4 (homologous to mouse Mip-1b)
• Small-inducible cytokine A4
• T-cell activation protein 2

Background

CCL4, also known as Macrophage Inflammatory Protein 1 beta (MIP-1 beta ), is a member of the CC or beta chemokine subfamily. CCL4 is expressed primarily by T cells, B cells, and monocytes after antigen or mitogen stimulation. The functional receptor for CCL4 has been identified as CCR5. Mature human CCL4 shares 77% and 80% aa sequence identity with mouse and rat CCL4, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCL4/MIP-1 beta Antibody (IC271A)(1)

Publication using IC271A

• I Lisovsky, S Kant, A Tremblay-M, G Isitman, Z Kiani, FP Dupuy, L Gilbert, J Bruneau, NH Shoukry, B Lebouché, NF Bernard Differential contribution of education through KIR2DL1, KIR2DL3, and KIR3DL1 to antibody-dependent (AD) NK cell activation and ADCC J. Leukoc. Biol., 2019-01-30;0(0):. 2019-01-30 [PMID: 30698860] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCL4
• Uniprot:
  • P13236()