CCR6 Antibody

Immunohistochemistry-Paraffin: CCR6 Antibody [NB100-713] - Human spleen

Immunohistochemistry-Paraffin: CCR6 Antibody [NB100-713] - Section of mouse spleen

Product Discontinued

CCR6 Antibody Summary

• Immunogen: Synthetic peptide: YFVSVNTSYYSVDSEMLLCSLQEVRQFSR, corresponding to amino acids 18-46 of Human CCR6.
• Localization: Integral membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CCR6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:37500
  • Flow Cytometry 2-6 ul/10^6 cells in 100ul
  • Immunohistochemistry 1:125
  • Immunohistochemistry-Paraffin 1:125
  • Western Blot 1:250
• Control:

  CCR6 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM KHPO4, 0.14M NaCl and 1.0 mg/ml BSA
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CCR6 Antibody

• BN-1
• CC-CKR-6
• CCR6
• CCR-6
• CD196 antigen
• CD196
• chemokine (C-C motif) receptor 6
• CKRL3
• CKR-L3
• CKR-L3G-protein coupled receptor 29
• CKRL3LARC receptor
• CMKBR6
• CMKBR6STRL22
• DRY6
• GPR29
• GPR29C-C CKR-6
• GPRCY4
• GPR-CY4
• GPRCY4DRY-6
• lymphocyte, 22
• STRL22

Background

CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein coupled receptors. It is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP 3 alpha). This receptor has been shown to be important for B lineage maturation and antigen driven B cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. CCR6 has been reported to be expressed in intestine, colon, liver, lymph node, spleen, lung, blood, umbilical cord, placenta, skin, and pancreas. It has also been reported to be expressed in lymphocytes, T cells, and B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR6 Antibody (NB100-713)(2)

Publications using NB100-713

• Zhang Y, Xia Q, Li Y et al. CD44 Assists the Topical Anti-Psoriatic Efficacy of Curcumin-Loaded Hyaluronan-Modified Ethosomes: A New Strategy for Clustering Drug in Inflammatory Skin Theranostics 2019-01-01 [PMID: 30662553] (WB, Mouse)
• Du J, Seno A, Sasada S et al. Upregulated CCL20 and CCR6 in Cancer Stem Cells Converted from Mouse iPS Cells Journal of Research in Medical and Dental Science 2020-01-01 (FLOW, Mouse)

FAQs for CCR6 Antibody (NB100-713). (Showing 1 - 1 of 1 FAQs).

1. I am looking for an anti-bovine CCR6 antibody and I see your antibody below was raised in goat so may cross react. Do you know if it can be used in cattle for flow cytometry? CCR6 Antibody (NB100-713)
   • Unfortunately, the species that the antibody is made in is no indicator of the species it will react to. In this case, the immunogen for NB100-713 shares just 57% similarity with bovine CCR6, so this antibody will almost certainly not cross-react with bovine. In this case, the immunogens for NLS356 and NBP1-88565 shares 79% similarity with bovine and have the highest chance of cross-reactivity of any antibodies we carry, although this is still below the 85% that we generally prefer to recommend for cross-reactivity. These are the best two we currently have for your species.

Bioinformatics

• Gene Symbol: CCR6
• Entrez:
  • 1235(Human)
  • 12458(Mouse)
• Uniprot:
  • P51684()