CCR9 Antibody (112509) [Phycoerythrin]

MOLT-4 human acute lymphoblastic leukemia cell line was stained with Mouse Anti-Human CCR9 PE-conjugated Monoclonal Antibody (Catalog # FAB179P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 112509
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CCR9 Antibody (112509) [Phycoerythrin] Summary

• Additional Information: Clone 112509 was used by HLDA to establish CD designation
• Immunogen: BaF3 mouse pro-B cell line transfected with human CCR9
  Met1-Leu369
  Accession # P51686
• Specificity: Specifically stains human CCR9 transfectants and MOLT-4 cells. It does not react with the parental BaF3 cells or human CCR7-transfected BaF3 cells.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CCR9
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR9 Antibody (112509) [Phycoerythrin]

• C-C chemokine receptor type 9
• C-C CKR-9
• CC-CKR-9
• CCR9
• CCR-9
• CD199
• CDw199 antigen
• CDw199
• chemokine (C-C motif) receptor 9
• G protein-coupled receptor 28
• GPR28
• GPR-9-6G-protein coupled receptor 28

Background

CCR9 is a G protein-linked seven transmembrane domain cytokine receptor. CCR9 serves as a receptor for CCL25/TECK. It is expressed on mature and immature thymocytes and some peripheral T and B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CCR9 Antibody (FAB179P)(9)

Publications using FAB179P

• Sundstrom P, Ahlmanner F, Akeus P, Sundquist M, Alsen S, Yrlid U, Borjesson L, Sjoling A, Gustavsson B, Wong S, Quiding-Jarbrink M Human Mucosa-Associated Invariant T Cells Accumulate in Colon Adenocarcinomas but Produce Reduced Amounts of IFN-gamma. J Immunol, 2015-08-21;195(7):3472-81. 2015-08-21 [PMID: 26297765] (Flow Cytometry, Human)
• Dimova T, Brouwer M, Gosselin F, Tassignon J, Leo O, Donner C, Marchant A, Vermijlen D Effector Vgamma9Vdelta2 T cells dominate the human fetal gammadelta T-cell repertoire. Proc Natl Acad Sci U S A, 2015-01-23;112(6):E556-65. 2015-01-23 [PMID: 25617367] (Flow Cytometry, Human)
• Duluc D, Joo H, Ni L, Yin W, Upchurch K, Li D, Xue Y, Klucar P, Zurawski S, Zurawski G, Oh S Induction and activation of human Th17 by targeting antigens to dendritic cells via dectin-1. J Immunol, 2014-05-16;192(12):5776-88. 2014-05-16 [PMID: 24835401] (Flow Cytometry, Functional Assay, Human)
• Hosomi S, Oshitani N, Kamata N, Sogawa M, Okazaki H, Tanigawa T, Yamagami H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T Increased numbers of immature plasma cells in peripheral blood specifically overexpress chemokine receptor CXCR3 and CXCR4 in patients with ulcerative colitis. Clin. Exp. Immunol., 2010-11-19;163(2):215-24. 2010-11-19 [PMID: 21087446] (Flow Cytometry, Human)
• Dusseaux M, Martin E, Serriari N, Peguillet I, Premel V, Louis D, Milder M, Le Bourhis L, Soudais C, Treiner E, Lantz O Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells. Blood, 2010-11-17;117(4):1250-9. 2010-11-17 [PMID: 21084709] (Flow Cytometry, Human)
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010-02-17;184(6):2886-98. 2010-02-17 [PMID: 20164417] (Flow Cytometry, Human)
• Clay CC, Rodrigues DS, Harvey DJ, Leutenegger CM, Esser U Distinct chemokine triggers and in vivo migratory paths of fluorescein dye-labeled T Lymphocytes in acutely simian immunodeficiency virus SIVmac251-infected and uninfected macaques. J. Virol., 2005-11-01;79(21):13759-68. 2005-11-01 [PMID: 16227295] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• Clay CC, Rodrigues DS, Brignolo LL, Spinner A, Tarara RP, Plopper CG, Leutenegger CM, Esser U Chemokine networks and in vivo T-lymphocyte trafficking in nonhuman primates. J. Immunol. Methods, 2004-10-01;293(1):23-42. 2004-10-01 [PMID: 15541274] (Flow Cytometry, Primate - Macaca mulatta (Rhesus Macaque))
• Liu LY, Jarjour NN, Busse WW, Kelly EA Chemokine receptor expression on human eosinophils from peripheral blood and bronchoalveolar lavage fluid after segmental antigen challenge. J. Allergy Clin. Immunol., 2003-09-01;112(3):556-62. 2003-09-01 [PMID: 13679815] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CCR9
• Uniprot:
  • P51686()