CD117/c-kit Antibody (104D2)

Product Discontinued

CD117/c-kit Antibody (104D2) Summary

• Immunogen: Unfortunately, this information is considered to be commercially sensitive
• Localization: Type I membrane protein.
• Marker: Hematopoietic Stem Cell Marker
• Specificity: This recognises the human c-Kit. c-Kit is expressed by some haematopoietic stem cells (approx. 70% of CD34 +ve cells in normal bone marrow are c-Kit +ve), by some acute myeloid leukaemias and by mast cells.This is non-neutralising.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KIT
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:2-1:20
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: Flow Cytometry Use at a dilution of 1/2 - 1/20. Use 10ul of the suggested working dilution to label 106 cells in 100ul.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24664884)

Reactivity Notes

Cross reacts with Cynomolgus Monkey. Mouse reactivity reported in scientific literature (PMID: 24664884) Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD117/c-kit Antibody (104D2)

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

CD117 is a 145 kD immunoglobulin superfamily member, also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with it's ligand is critical for the development of hematopoietic stem cells. The 2B8 antibody does not block c-Kit activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD117/c-kit Antibody (NB600-765)(1)

Publication using NB600-765

• Wickham AM, Islam MM, Mondal D et al. Polycaprolactone-thiophene-conjugated carbon nanotube meshes as scaffolds for cardiac progenitor cells. J. Biomed. Mater. Res. Part B Appl. Biomater. 2014-03-24 [PMID: 24664884] (ICC/IF, FLOW, Mouse)

Bioinformatics

• Gene Symbol: KIT