CD11c Antibody (3.9) - BSA Free

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: 3.9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD11c Antibody (3.9) - BSA Free Summary

• Additional Information: Clone 3.9 was used by HLDA to establish CD designation.
• Immunogen: Rheumatoid synovial fluid cells and fibronectin purified human monocytes
• Localization: Type I membrane protein.
• Marker: Dendritic Cell Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1 ug per 1x10^6
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: Shown to inhibit adhesion (PMID: 22844116). This antibody is CyTOF ready.
• Theoretical MW: 150 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD11c Antibody (3.9) - BSA Free

• 95 alpha chain
• 95
• 95, alpha subunit
• CD11 antigen-like family member C
• CD11c antigen
• CD11c
• CD11Cleu M5, alpha subunit
• Integrin alpha X
• integrin alpha-X
• integrin, alpha X (antigen CD11C (p150), alpha polypeptide)
• integrin, alpha X (complement component 3 receptor 4 subunit)
• ITGAX
• Leu M5
• Leukocyte adhesion glycoprotein p150
• Leukocyte adhesion receptor p150
• leukocyte surface antigen p150
• myeloid membrane antigen, alpha subunit
• p150 95 integrin alpha chain
• p150,95 alpha
• SLEB6

Background

CD11c (integrin alpha X chain protein) combines with the beta 2 chain (ITGB2) to form a leukocyte specific integrin referred to as inactivated C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. The CD11c antigen is a marker for hairy cell leukaemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGAX
• Entrez:
  • 3687(Human)