CD24- CD44+ Breast Cancer Stem Cell Isolation Kit

Flow Cytometry: CD24- CD44+ Breast Cancer Stem Cell Isolation Kit [MAGH111-NOV] - A small CD24low/-CD44+ population (A, upper-left quadrant) was isolated using this MagCellect Human Breast Cancer Stem Cells Isolation Kit (B). A histogram profiling the enrichment of CD24low/-CD44+ cells (shaded histogram) is shown (C)

Flow Cytometry: CD24- CD44+ Breast Cancer Stem Cell Isolation Kit [MAGH111-NOV] - A small population of CD24 low /- CD44+ cells was isolated from MCF-7 human breast adenocarcinoma cells using the MagCellect. CD24 low /- CD44+ cells (upper left quadrant), before (A) and after (B) enrichment, were detected by double-staining with APC-conjugated Mouse Anti-Human CD24 and PE-conjugated Mouse Anti-Human CD44 Detection Antibodies (both provided in the kit). A histogram profiling the enrichment of CD24 low/- cells (filled histogram) from the original cell population (open histogram) is shown (C).

Product Discontinued

CD24- CD44+ Breast Cancer Stem Cell Isolation Kit Summary

• Description: The MagCellect Human CD24- CD44+ Breast Cancer Stem Cells Isolation Kit is designed to isolate putative human breast cancer stem cells via a positive selection principle. The resulting cell preparation is highly enriched for CD24low/-CD44+ cells. Purity of recovered CD24low/-CD44+ cells varies depending on the preparation.
  
  Features
  • Isolation by positive and negative selection improves purity of recovered cells
  • Isolation based on two markers increases the purity of recovered cells
  • Includes antibodies for analysis of the final cell population purity
• Kit Type: Isolation Kit

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

Cancer stem cells are a rare population of self-renewing cells found in tumors and in cancer cell lines. Methods that use positive or negative selection to enrich for cancer stem cells can lead to poor recovery and low cell purity as well as the loss of valuable tissue samples and resources. MagCellect products are designed for the isolation of cells in a "liquid phase". MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.