CD25/IL-2R alpha Antibody (IL2R.1)

Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (IL2R.1) [NB600-564] - Formalin fixed paraffin embedded human tonsil stained with CD25 antibody (NB600-564).

Immunohistochemistry-Paraffin: IL2RA/CD25 Antibody (IL2R.1) [NB600-564] - Formalin fixed paraffin embedded human tonsil stained with CD25, using ABC method.

Immunohistochemistry-Paraffin: CD25/IL-2 R alpha Antibody (IL2R.1) [NB600-564] - Human Tonsil

Product Discontinued

CD25/IL-2R alpha Antibody (IL2R.1) Summary

• Immunogen: BALB/C mice were injected with recombinant human interleukin-2 receptor.
• Localization: Cell Membrane
• Specificity: This antibody is specific to a 55 kDa protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cells, HTLV-1 or HTLV-2 positive T cell lines. It is moderately expressed on activated B cells, NK-cells, and macrophages.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2RA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:25-1:75
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:25-1:75
• Application Notes: IHC-P: recommended pretreatment of EDTA buffer, pH 8.0. Recommended incubation time of 30 min at RT. Use on frozen sections was reported in scientific literature (PMID: 22665223). Positive control for IHC is Tonsil tissue. This antibody has also been reported to work in ICC/IF in mouse (PMID:22009917).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for CD25/IL-2R alpha Antibody (IL2R.1)

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolyisis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD25/IL-2R alpha Antibody (NB600-564)(7)

Publications using NB600-564

• Oami T, Abtahi S, Shimazui T et al. Claudin-2 upregulation enhances intestinal permeability, immune activation, dysbiosis, and mortality in sepsis. Proceedings of the National Academy of Sciences of the United States of America 2024-02-29 [PMID: 38412124]
• Bourgeois JR, Kalyanasundaram G, Figueroa C et al. A semi-automated brain atlas-based analysis pipeline for c-Fos immunohistochemical data J Neurosci Methods 2020-10-20 [PMID: 33091429] (IHC-P, Human)
• Grati M, Yan D, Raval MH et al. Myo3a Causes Human Dominant Deafness And Interacts With Protocadherin 15-Cd2 Isoform Hum. Mutat. 2016-02-03 [PMID: 26841241] (ICC/IF)
• Liang Y, Gao H, Lin Sy et al. Mcph1/Brit1 deficiency promotes genomic instability and tumor formation in a mouse model Oncogene et al. 2014-11-03 [PMID: 25362854] (IHC-Fr, Mouse)
  
  Details:
  CD25/IL-2 R alpha antibody used for IHC-Fr on thymus tissues from wild-type (WT) mice and primary lymphomas from Mcph1+/+p53-/-, Mcph1+/-p53-/-, and Mcph1-/-p53-/- mice. The antibody was used as the molecular marker for lymphocyte development and normal thymus was considered as control (Figure S8)
• Sorrentino D, Avellini C, Geraci M, Vadala S. Pre-clinical Crohns disease: Diagnosis, treatment and six year follow-up. J Crohns Colitis. 2014-01-08 [PMID: 24411923] (IHC-P, Human)
  
  Details:
  Intestinal/colon tissue biopsies, Fig 2.
• Campbell-Thompson ML, Heiple T, Montgomery E et al. Staining protocols for human pancreatic islets. J Vis Exp 2012-05-01 [PMID: 22665223] (IHC-Fr, Human)
• Joo, H J, Kim, H S, Choi, Y S et al. Detection of prostaglandin E2-induced dendritic cell migration into the lymph nodes of mice using a 1.5 T clinical MR scanner NMR Biomed 2012-01-01 [PMID: 22009917] (IF/IHC, ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: IL2RA
• Entrez:
  • 3559(Human)
• Uniprot:
  • P01589()